From 2009.igem.org
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| - | ===21.07.09===
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| - | One useful website to know the restriction sites of enzymes:
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| - | <br>http://www.genscript.com/cgi-bin/products/enzyme.cgi?op=all_ez
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| - | The restriction site used were:
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| - | <br>EcoRI GAATTC
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| - | <br>XbaI TCTAGA
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| - | <br>SpeI ACTAGT
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| - | <br>PsiI TTATAA
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| - | Design the primers for the 2 step-PCR:
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| - | the first step introduces the LacI promoter and the RBS upstream the LovTAP gene with the Forward primer, whereas the Reverse introduces the Term downstream.
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| - | In the second step we only introduce the E-X restriction sites upstream and the SP downstream.
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| | ===22.07.09=== | | ===22.07.09=== |
Revision as of 07:57, 28 July 2009
Cloning strategy
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