Team:EPF-Lausanne/Notebook/Wet Lab

From 2009.igem.org

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===27.07.09===
 
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LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert.
 
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[http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)] was digested, runned on a gel (which displayed the correct band) and extracted with the gel extraction kit, this step didn't worked well.
 
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LOVTAP gene have been than digested, purified and runned on a gel.
 
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We already had a digested [http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)].
 
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Finally we ligated overnight at 4°C the digested LOVTAP in both [http://partsregistry.org/Part:BBa_B0015 terminators], the one digested-extracted today and the one already digested-extracted.
 
===28.07.09===
===28.07.09===

Revision as of 08:02, 28 July 2009

Contents

Wet Lab

28.07.09

29.07.09

30.07.09

31.07.09

August