Virginia Commonwealth/21 May 2009
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#Make stock of electrocompetent ''E. coli'' K-12 | #Make stock of electrocompetent ''E. coli'' K-12 | ||
#Transform ''E. coli'' with the following: | #Transform ''E. coli'' with the following: | ||
- | ##BBa_I13521 (RPF PRET | + | ##BBa_I13521 (RPF PRET) - located in plate 2, well 6O (AmpR) |
- | ##BBa_I13522 (GRP PRET | + | ##BBa_I13522 (GRP PRET) located in plate 2, well 8A (AmpR) |
- | ##BBa_E0840 (GFP RET | + | ##BBa_E0840 (GFP RET) - located in plate 1, well 12O (AmpR) |
- | ##BBa_R0040 (Promoter) - located in plate 1, well 6I | + | ##BBa_R0040 (Promoter) - located in plate 1, well 6I (AmpR) |
- | ##pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in palte 1, well 13A | + | ##pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in palte 1, well 13A (KanR) |
- | ##BBa_E0240 (GFP RET, AmpR) - located in plate 1, well | + | ##BBa_E0240 (GFP RET) - located in plate 1, well 12M) (AmpR) |
+ | ##BBa_J23101 (Promoter) - located in plate 1, well 18E (AmpR) | ||
===Results=== | ===Results=== |
Revision as of 15:11, 21 May 2009
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Thursday 21 May 2009
Tasks
- Make stock of electrocompetent E. coli K-12
- Transform E. coli with the following:
- BBa_I13521 (RPF PRET) - located in plate 2, well 6O (AmpR)
- BBa_I13522 (GRP PRET) located in plate 2, well 8A (AmpR)
- BBa_E0840 (GFP RET) - located in plate 1, well 12O (AmpR)
- BBa_R0040 (Promoter) - located in plate 1, well 6I (AmpR)
- pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in palte 1, well 13A (KanR)
- BBa_E0240 (GFP RET) - located in plate 1, well 12M) (AmpR)
- BBa_J23101 (Promoter) - located in plate 1, well 18E (AmpR)