Revision as of 13:46, 2 August 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Individual parts

Short description	Resistance	Status	Location	Additional notes
Ribokey	A/A	ordered at Geneart
antikey	A/A	ordered at geneart
lock	Ap	ordered at geneart
Terminator	Ak	Available here	-80
RBS	Ap	Available here	-80
promotor	Ap	Available here	-80
Sam8		send from Edinburgh
Sam5		send from Edinburgh
comt	A/A	send from Edinburgh
fcs	ap	received from Edinburgh	-20
ech	ap	received from Edinburgh	-20
VirA receptor		primers ordered		This is purified from Agrobacterium temefaciens
VirG activator		primers ordered		This is purified from Agrobacterium tumefaciens
RpoA		primers ordered		This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
VirB promoter region		primers ordered		This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
YcgF/E promotor		region purified from E.coli strain MC4100		blue light promotor region from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1
YcgF/E promotor		primers ordered		exact blue light promotor from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1

Short description	Status	Additional notes
VirB promotor + key	Biobrick in registry
Blr promotor + key	Biobrick in registry
Vanillin receptor	Biobrick in registry
Vanillin synthesis	Biobrick in registry	5 paths in pathway never tested together in E. Coli
Short Vanillin synthesis	Biobrick in registry	Short version containing the last 2 genes of the pathway, these have been tested in E. Coli
Short Vanillin synthesis II	Biobrick in registry	Short version containing the first 3 parts of the pathway, these have not been tested in E. Coli

@@ Line 37: / Line 37: @@
 | {{kulpart|BBa_K238011|icon}}|| VirB promoter region  || || primers ordered || || This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
 |-align="center"
-| {{kulpart|BBa_K238000|icon}}|| YcgF/E promotor || -20 || region purified, actual promoter identified|| || blue light promotor from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]
+| {{kulpart|BBa_K238000|icon}}|| YcgF/E promotor || || region purified from E.coli strain MC4100|| || blue light promotor region from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]
+|-align="center"
+| {{kulpart|BBa_K238013|icon}}|| YcgF/E promotor || || primers ordered|| || exact blue light promotor from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]
 |}