EPF-Lausanne/1 August 2009
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+ | <input type="button" name="lien" value="30 July 2009" | ||
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+ | <input type="button" name="lien" value="01 August 2009" | ||
+ | onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/1_August_2009'"> | ||
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+ | <font size="6" color="#007CBC"><i>31 July 2009</i></font> | ||
+ | </center></html> | ||
+ | <br> | ||
+ | ---- | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | People in the lab will live at the rythm of the bacteria this week end :) | ||
+ | |||
==Wet Lab== | ==Wet Lab== | ||
+ | |||
+ | We transformed competent bacteria with our different ligation products from the previous day: | ||
+ | - neg control with vector only (Chl.) | ||
+ | - neg control without any DNA (Amp.) | ||
+ | - LacI-RBS from the gel extraction (Amp.) | ||
+ | - LacI-RBS not gel-extracted (Amp.) | ||
+ | - LacI-RBS-death cassette (Chl.) | ||
+ | |||
+ | The bacteria were left to grow overnight at 37°C on the LB-agar plates. | ||
+ | |||
+ | ==People in the lab== | ||
+ | |||
+ | Christian, Mélanie, Nath, Basile, Gab | ||
+ | |||
+ | |||
+ | <html><center><a href="https://2009.igem.org/EPF-Lausanne/30_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a> | ||
+ | <a href="https://2009.igem.org/EPF-Lausanne/1_August_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html> | ||
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Revision as of 13:50, 2 August 2009
Contents |
People in the lab will live at the rythm of the bacteria this week end :)
Wet Lab
We transformed competent bacteria with our different ligation products from the previous day: - neg control with vector only (Chl.) - neg control without any DNA (Amp.) - LacI-RBS from the gel extraction (Amp.) - LacI-RBS not gel-extracted (Amp.) - LacI-RBS-death cassette (Chl.)
The bacteria were left to grow overnight at 37°C on the LB-agar plates.
People in the lab
Christian, Mélanie, Nath, Basile, Gab