EPF-Lausanne/1 August 2009

From 2009.igem.org

(Difference between revisions)
Line 7: Line 7:
<form action="input_button.htm">
<form action="input_button.htm">
<p align="right">
<p align="right">
-
<input type="button" name="lien" value="30 July 2009"
+
<input type="button" name="lien" value="31 July 2009"
-
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/30_July_2009'">
+
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/31_July_2009'">
-
<input type="button" name="lien" value="01 August 2009"  
+
<input type="button" name="lien" value="02 August 2009"  
-
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/1_August_2009'">
+
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/2_August_2009'">
</p>
</p>
</form>
</form>
Line 17: Line 17:
<html><center>
<html><center>
-
<font size="6" color="#007CBC"><i>31 July 2009</i></font>  
+
<font size="6" color="#007CBC"><i>1 August 2009</i></font>  
</center></html>
</center></html>
<br>
<br>
Line 24: Line 24:
<br>
<br>
-
People in the lab will live at the rythm of the bacteria this week end :)
 
==Wet Lab==
==Wet Lab==
-
We transformed competent bacteria with our different ligation products from the previous day:
+
We checked the plates from the transformation of the previous day: only 7 clones grew on the LacI-RBS (not gel extracted) plate. We did a colony PCR with each of the clones that grew to check which clones potentially contained the desired fragment. To check this, we then ran agarose gel with samples of each of the PCRs. The result: 3 of the clones gave a fragment of the expected length!!!
-
- neg control with vector only (Chl.)
+
What was left of these clones was then put into liquid culture and left to incubate at 37°C.
-
- neg control without any DNA (Amp.)
+
-
- LacI-RBS from the gel extraction (Amp.)
+
-
- LacI-RBS not gel-extracted (Amp.)
+
-
- LacI-RBS-death cassette (Chl.)
+
-
 
+
-
The bacteria were left to grow overnight at 37°C on the LB-agar plates.
+
==People in the lab==
==People in the lab==
-
Christian, Mélanie, Nath, Basile, Gab
+
Nath, Basile, Gab

Revision as of 13:57, 2 August 2009



Contents

1 August 2009




Wet Lab

We checked the plates from the transformation of the previous day: only 7 clones grew on the LacI-RBS (not gel extracted) plate. We did a colony PCR with each of the clones that grew to check which clones potentially contained the desired fragment. To check this, we then ran agarose gel with samples of each of the PCRs. The result: 3 of the clones gave a fragment of the expected length!!! What was left of these clones was then put into liquid culture and left to incubate at 37°C.

People in the lab

Nath, Basile, Gab



Retrieved from "http://2009.igem.org/EPF-Lausanne/1_August_2009"