Categories :

Home Team Sponsors Parts Submitted to Registry Image Gallery Leave a Message!

Project :

Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

Notebook :

Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10

Team Logistics :

Protocols Stock List Research Shared Links and Help

Violacein Pigment

Introduction

The Violacein pigment is produced from L-tryptophan via a pathway involving four enzymes, vioA-D. This scheme is shown below:

From P.R. August, T.H. Grossman, C. Minor, M.P. Draper, I.A. MacNeil, J.M. Pemberton, K.M. Call, d. Holt, and M. S. Osbourne, Sequence Analysis and Functional Characterization of the Violacein Biosynthetic Pathway from Chromobacterium violaceum, J. Mol. Microbiol. Biotechnol. (2000) 2(4): 513-519. [http://www.horizonpress.com/jmmb/v2/v2n4/26.pdf]

Further, as module 5 is Aqua, controlling VioA, VioB, VioC, and VioD allows us to generate two different pigments, aqua and violet.

Research by previous iGem teams

Previous biobricks:

Action plan of our team

Our action plan is as follows:

1.

Test activity of X

Mapping of Biobrick X: (image)

Construct composite Biobrick X

Plan mapping of X:

(image)

Test colour changes by X

Characterisation of Biobricks and colour output

Test compatibility with other biobricks