"
Team:Groningen/Notebook/7 August 2009
From 2009.igem.org
(Difference between revisions)
m (→GVP Cluster) |
(→GVP Cluster) |
||
| Line 19: | Line 19: | ||
* From each tube 4mL of culture was collected in a 2.0mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded. | * From each tube 4mL of culture was collected in a 2.0mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded. | ||
* Plasmids were eluted with 20μL MQ and stored in the fridge | * Plasmids were eluted with 20μL MQ and stored in the fridge | ||
| + | |||
| + | '''Concentration of Plasmids''' | ||
| + | |||
| + | <div style="text-align: left;"> <font size="1" style="color:grey"> | ||
| + | <left> | ||
| + | {|cellpadding="2" cellspacing="1" border="4" | ||
| + | |'''Component''' | ||
| + | |'''Description''' | ||
| + | |'''Part or Accession #''' | ||
| + | |'''Base Pairs (bp)''' | ||
| + | |'''Plasmid (backbone)''' | ||
| + | |- | ||
| + | |GVP | ||
| + | |Gas Vesicle Proteins | ||
| + | |[http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | ||
| + | |6064 | ||
| + | |[http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div align="left"> | ||
===Transporters=== | ===Transporters=== | ||
Revision as of 08:45, 7 August 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
|
|---|
Wet
GVP Cluster
- → TODO isolate plasmids from overnight precultures
- → TODO perform a restriction on isolated plasmid (should result in 6000 and 3000bp fragments)
- → TODO plate on cultures for pure colony over the weekend
- → TODO cut pSB3K3 plasmid and GVP containing plasmid for ligation
- → TODO purify wanted fragments
Plasmid Purification
Plasmid isolation was performed on the cultures of E.coli TOP10 containing plasmids [http://partsregistry.org/wiki/index.php?title=Part:pSB1AC3 pSB1AC3] with high, medium and low constitutive promoters and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I750016 GVP] with the "Sygma-Aldrich™ [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/plasmid-miniprep-kit.html GenElute™] Plasmid Miniprep Kit".
- From each tube 4mL of culture was collected in a 2.0mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded.
- Plasmids were eluted with 20μL MQ and stored in the fridge
Concentration of Plasmids
<left>
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) |
| GVP | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] |
Transporters
Metal Accumulation
Vectors
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
