From 2009.igem.org
(Difference between revisions)
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- | |? | + | |B-1 |
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- | |? | + | |B-2 |
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| |EcoRI/PstI | | |EcoRI/PstI |
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| |2.00 | | |2.00 |
| |2.44 | | |2.44 |
- | |? | + | |A-4 |
| |EcoRI/PstI | | |EcoRI/PstI |
| |- | | |- |
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| |1.92 | | |1.92 |
| |2.40 | | |2.40 |
- | |? | + | |A-5 |
| |EcoRI/PstI | | |EcoRI/PstI |
| |- | | |- |
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| |1.95 | | |1.95 |
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| |EcoRI/PstI | | |EcoRI/PstI |
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| |1.96 | | |1.96 |
| |2.43 | | |2.43 |
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| |EcoRI/PstI | | |EcoRI/PstI |
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| |1.92 | | |1.92 |
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| |1.94 | | |1.94 |
| |2.37 | | |2.37 |
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| |EcoRI/PstI | | |EcoRI/PstI |
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| |1.95 | | |1.95 |
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| |EcoRI/PstI | | |EcoRI/PstI |
| |- | | |- |
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| |1.91 | | |1.91 |
| |2.31 | | |2.31 |
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| |EcoRI/PstI | | |EcoRI/PstI |
| |} | | |} |
Latest revision as of 09:39, 11 August 2009
Wet
GVP Cluster
- → DONE isolate plasmids from overnight precultures
- → DONE perform a restriction on isolated plasmid (should result in 6000 and 3000bp fragments)
- → TODO plate on cultures for pure colony over the weekend
- → TODO cut pSB3K3 plasmid and GVP containing plasmid for ligation
- → TODO purify wanted fragments
Plasmid Purification
Plasmid isolation was performed on the cultures of E.coli TOP10 containing plasmids [http://partsregistry.org/wiki/index.php?title=Part:pSB1AC3 pSB1AC3] with high, medium and low constitutive promoters and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I750016 GVP] with the "Sygma-Aldrich™ [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/plasmid-miniprep-kit.html GenElute™] Plasmid Miniprep Kit".
- From each tube 4mL of culture was collected in a 2.0mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded.
- Plasmids were eluted with 20μL MQ and stored in the fridge
Concentration of Plasmids
Plasmid
| Conc. ng/μL
| 260/280
| 260/230
| -20 box (michael
| Restriction Control
|
pSB3K3-H
| 25.0
| ?
| ?
| B-1
| ?
|
pSB3K3-M
| 32.6
| ?
| ?
| B-2
| ?
|
pSB3K3-L
| 33.9
| ?
| ?
| B-3
| ?
|
GVP (biobrick)
| 484.0
| ?
| ?
| E-5
| EcoRI/PstI
|
pSB1AC3-H-GVP-amp
| 106.6
| 2.00
| 2.44
| A-4
| EcoRI/PstI
|
pSB1AC3-M-GVP-amp
| 165.2
| 1.92
| 2.40
| A-5
| EcoRI/PstI
|
pSB1AC3-L-GVP-amp
| 155.0
| 1.95
| 2.44
| A-6
| EcoRI/PstI
|
pSB1AC3-H-GVP-amp-conc.
| 116.3
| 1.96
| 2.43
| Not Stored
| EcoRI/PstI
|
pSB1AC3-M-GVP-amp-conc.
| 108.7
| 1.95
| 2.46
| Not Stored
| ?
|
pSB1AC3-L-GVP-amp-conc.
| 302.8
| 1.88
| 2.45
| Not Stored
| ?
|
pSB1AC3-H-GVP-amp/chl-conc.
| 257.1
| 1.92
| 2.41
| A-7
| EcoRI/PstI
|
pSB1AC3-M-GVP-amp/chl-conc.
| 224.7
| 1.94
| 2.37
| A-8
| EcoRI/PstI
|
pSB1AC3-L-GVP-amp/chl-conc.
| 166.1
| 1.95
| 2.44
| A-9
| EcoRI/PstI
|
pSB1AC3-L-GVP-amp/chl-oud
| 223.9
| 1.91
| 2.31
| Not Stored
| EcoRI/PstI
|
GVP+Metal dependent promotors
Plasmids suspected to be positive for the metal dependent promotors in front of the GVP cluster were restricted for 1 h @ 37 °C using [http://fermentas.com/catalog/re/fastnoti.htm NotI]. No enzyme was present anymore to do restriction of sample 4 1x (Paul code).
Samples were put on 1% (TBE) agarose gel with a 1 kB DNA marker. Postive signals should be seen a ~6000 & ~2000, negative (first lane after marker) at ~7000 & ~2500.
Transporters
GlpF
The GlpF PCRs done yesterday (PCR 1 and2) were run on gel (lanes 2,3). Also the restriction done yesterday in order to check the ligations are shown on the gel below. The restriction was performed with HaeII for details see the notebook of 06-08-09.
a band of 105 was expected for GlpF PCR1 which could not be seen on the above 1% agarose gel. Therefor 1uL of PCR1 (with 4 uL MQ and 1 uL 6x orange loading dye) was loaded to a 2% gel as shown below (arrow), since the amount of loaded DNA is low the band is not very dark.
Metal Accumulation
- 2nd PCR to amplify SmtA and GST-SmtA
- Use pGEX-3X-GST-SmtA to amplify SmtA-GST and SmtA
- Use pET29a-SmtA to amplify SmtA
- Program: MT NIENKE (Left PCR machine)
- Globally: 10cycles touchdown from 65-50°C, 25 cycles with Tm gradient from 60-50°C (4steps), elongation time of 1:20.
- Mix
- MM NH4 21uL
- Primer fw [10mM] 1uL
- Primer rev [10mM] 1uL
- Vector 1uL
- HomeTaq pol 1uL
- Run program o/n
- Check on gel
- No bands seen on gel, except for SmtA-GST nr 4 (highest Tm) which gave a band around 900bp... Which is too small for SmtA-GST...
- --> The tubes where unfortunately not put in the correct position, so the Tm at the gradient step was ~45-55°C....
- Transform E. coli with pGB68 (mymT)
- Use pGB68 from filter resuspended in 40uL MQ.
- As neg control use pSB1AC3 (incl ccdB), as pos control use pSB1AC3-low promoter.
- Mix 2-4uL of vector with 50uL chemically competent cells (prepared 31 aug)
- Transform using heat-shock @ 37°C
Vectors
Dry
We (Jasper and Annelies) looked at using figure 1 from Singh2008. So far it looks like it shows problems similar to those we encountered with figure 3A from Kostal2004. Specifically, if we plug in the constants for GlpF we determined earlier it looks like either the export rate or the concentration at which it reaches half its maximum rate should be negative... We weren't able to get Mathematica to use Minimize on our cost function, so we couldn't try fitting all four parameters at once, we should try this with figure 3A from Kostal2004 as soon as Klaas Bernd is back.
April
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M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
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May
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M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_May_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_May_2009 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
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June
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M | T | W | T | F | S | S
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_June_2009 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
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[http://2009.igem.org/Team:Groningen/Notebook/8_June_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
|
[http://2009.igem.org/Team:Groningen/Notebook/15_June_2009 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_June_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_June_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_June_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_June_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_June_2009 21]
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[http://2009.igem.org/Team:Groningen/Notebook/22_June_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_June_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_June_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_June_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_June_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
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July
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Team:Groningen/Notebook/1_July_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_July_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
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[http://2009.igem.org/Team:Groningen/Notebook/6_July_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_July_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_July_2009 8]
| [http://2009.igem.org/Team:Groningen/Notebook/9_July_2009 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
|
[http://2009.igem.org/Team:Groningen/Notebook/13_July_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_July_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_July_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_July_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
|
[http://2009.igem.org/Team:Groningen/Notebook/20_July_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_July_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_July_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_July_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_July_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_July_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
|
[http://2009.igem.org/Team:Groningen/Notebook/27_July_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_July_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_July_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_July_2009 30]
| [http://2009.igem.org/Team:Groningen/Notebook/31_July_2009 31]
|
|
August
|
M | T | W | T | F | S | S
|
|
|
|
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_August_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
|
[http://2009.igem.org/Team:Groningen/Notebook/3_August_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_August_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_August_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_August_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_August_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_August_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
|
[http://2009.igem.org/Team:Groningen/Notebook/10_August_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_August_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_August_2009 12]
| [http://2009.igem.org/Team:Groningen/Notebook/13_August_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_August_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_August_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_August_2009 16]
|
[http://2009.igem.org/Team:Groningen/Notebook/17_August_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_August_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_August_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_August_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_August_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_August_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_August_2009 23]
|
[http://2009.igem.org/Team:Groningen/Notebook/24_August_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_August_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_August_2009 26]
| [http://2009.igem.org/Team:Groningen/Notebook/27_August_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_August_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_August_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_August_2009 30]
|
[http://2009.igem.org/Team:Groningen/Notebook/31_August_2009 31]
|
|
September
|
M | T | W | T | F | S | S
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_September_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_September_2009 2]
| [http://2009.igem.org/Team:Groningen/Notebook/3_September_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_September_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_September_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_September_2009 6]
|
[http://2009.igem.org/Team:Groningen/Notebook/7_September_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_September_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_September_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_September_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_September_2009 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
|
[http://2009.igem.org/Team:Groningen/Notebook/14_September_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_September_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_September_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_September_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_September_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_September_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
|
[http://2009.igem.org/Team:Groningen/Notebook/21_September_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_September_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_September_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_September_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
|
[http://2009.igem.org/Team:Groningen/Notebook/28_September_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_September_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_September_2009 30]
|
|
October
|
M | T | W | T | F | S | S
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_October_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
|
[http://2009.igem.org/Team:Groningen/Notebook/5_October_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_October_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
|
[http://2009.igem.org/Team:Groningen/Notebook/19_October_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
|
|
November
|
M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_November_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_November_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
|
|