Team:Newcastle/Labwork/10 August 2009

From 2009.igem.org

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====Sample1====
====Sample1====
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  The concentration of DNA: 124.6 ng/uL
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  The concentration of DNA: 829.6 ng/uL
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  The ratio of DNA concentration to protein concentration(260/280): 1.84
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  The ratio of DNA concentration to protein concentration(260/280): 1.88
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  The ratio of protein concentration to RNA concentration(260/230): 1.73
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  The ratio of protein concentration to RNA concentration(260/230): 2.33
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[[Image:Team_Newcastle_iGEM_2009_08_10_Sample_1.png‎|550px]]
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[[Image:Team_Newcastle_iGEM_2009_08_10_Sample_1.png‎|500px]]
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====Sample2====
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The concentration of DNA: 601.1 ng/uL
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The ratio of DNA concentration to protein concentration(260/280): 1.90
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The ratio of protein concentration to RNA concentration(260/230): 2.11
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[[Image:Team_Newcastle_iGEM_2009_08_10_Sample_2.png‎|500px]]
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[[Image:Team_Newcastle_iGEM_2009_08_10_Sample_2.png‎|550px]]
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==Chassis team==
==Chassis team==

Revision as of 15:40, 10 August 2009

Contents

Lab Session 10/08/09

Stochastic Switch Team

Today we pepared gfp-rrnb plasmids using midiprep. We followed GenElute's midiprep protocol.

We had two E.coli cultures inoculated with gfp-rrnb left overnight. Although they had teh sampels, we treated them individually.

We centrifuged the tubes at 16000rpm for 30 minutes before adding ethanol to rinse the DNAs. We then centriguged the tubes again for 10 minutes at 16000rpm.

We evaporated the tubes fro 5 minutes at the end.

Results

Sample1

The concentration of DNA: 829.6 ng/uL
The ratio of DNA concentration to protein concentration(260/280): 1.88
The ratio of protein concentration to RNA concentration(260/230): 2.33

Team Newcastle iGEM 2009 08 10 Sample 1.png

Sample2

The concentration of DNA: 601.1 ng/uL
The ratio of DNA concentration to protein concentration(260/280): 1.90
The ratio of protein concentration to RNA concentration(260/230): 2.11

Team Newcastle iGEM 2009 08 10 Sample 2.png

Chassis team

  • Created germination solution without lysozyme.
  • Borrowed Em from Colin Harwood's lab.
  • Created a new MgCl Stock solution, as old one had bits of matter floating in it.
  • Created KPh stock solution.
  • Autoclaved solutions.
  • Made 250ml LB and 250ml H20, autoclaved.
  • Inoculated LB with Em and Kan, then poured plates.



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