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Revision as of 14:19, 12 August 2009 (view source) Bussingkm (Talk | contribs) (→Wednesday 12 August 2009) ← Older edit		Revision as of 14:22, 12 August 2009 (view source) Bussingkm (Talk | contribs) (→Wetlab) Newer edit →
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	====Wetlab====		====Wetlab====
-	* 1	+	* 1 I don't think that the no growth constructs were not successful.  The last ligation reaction cleaned out the PCR product for D1-9.  Will perform another PCR reaction today so the ligation can be done again.
	* 2		* 2

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Revision as of 14:22, 12 August 2009

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October M T W T F S S       [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4] [http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11] [http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents 1 Wednesday 12 August 2009 1.1 Results 1.2 Tasks 1.2.1 Wetlab

Wednesday 12 August 2009

Results

growth results

Construct	Growth	Flourescence!
D1+BBa_J23101+pSB1C3	No Growth	N/A
D2+BBa_J06702+pSB1C3	No Growth	N/A
D3+BBa_J06702+pSB1C3	One Colony	Minimal at Best
D4+BBa_J06702+pSB1C3	No Growth	N/A
D5+BBa_J06702+pSB1C3	Three Colonies	No Flourescence
D6+BBa_J06702+pSB1C3	One Colony	No Flourescence
D7+BBa_J06702+pSB1C3	One Colony	Possible
D8+BBa_J06702+pSB1C3	No Growth	N/A
D9+BBa_J06702+pSB1C3	Three Colonies	No Flourescence
Positive Control	Lawn	No Flourescence
Negative Control	No Growth	N/A

I will wait for fluorescence to pick colonies.

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Tasks

• 1
• 2

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Wetlab

• 1 I don't think that the no growth constructs were not successful. The last ligation reaction cleaned out the PCR product for D1-9. Will perform another PCR reaction today so the ligation can be done again.
• 2

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