Virginia Commonwealth/12 August 2009

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====Wetlab====
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* 1 I don't think that the no growth constructs were not successful.  The last ligation reaction cleaned out the PCR product for D1-9.  Will perform another PCR reaction today so the ligation can be done again.  
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''Kevin and Adam''
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* I don't think that the no growth constructs were not successful.  The last ligation reaction cleaned out the PCR product for D1-9.  Will perform another PCR reaction today so the ligation can be done again.  
** Purified PCR product did not show up on gel.  I will redo reaction tomorrow. [[User:Bussingkm|Bussingkm]] 22:26, 12 August 2009 (UTC)
** Purified PCR product did not show up on gel.  I will redo reaction tomorrow. [[User:Bussingkm|Bussingkm]] 22:26, 12 August 2009 (UTC)
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''Maria and Afton''
''Maria and Afton''

Latest revision as of 18:33, 13 August 2009

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[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents

Wednesday 12 August 2009

Results

growth results
ConstructGrowthFlourescence!
D1+BBa_J23101+pSB1C3 No Growth N/A
D2+BBa_J06702+pSB1C3 No Growth N/A
D3+BBa_J06702+pSB1C3 One Colony Minimal at Best
D4+BBa_J06702+pSB1C3 No Growth N/A
D5+BBa_J06702+pSB1C3 Three Colonies No Flourescence
D6+BBa_J06702+pSB1C3 One Colony No Flourescence
D7+BBa_J06702+pSB1C3 One Colony Possible
D8+BBa_J06702+pSB1C3 No Growth N/A
D9+BBa_J06702+pSB1C3 Three Colonies No Flourescence
Positive Control Lawn No Flourescence
Negative Control No Growth N/A

I will wait for fluorescence to pick colonies.

Maria and Afton

  • Results of transformation were not good
Name Plate Growth Observation
J23100 w/1C3,702 Cm no growth
J23102 w/1C3,702 Cm no growth
J23103 w/1C3,702 Cm no growth
J23104 w/1C3,702 Cm no growth
  • A gel will be run today on the digests to see how they look.
  • Plates will be kept for a few more days in case the bacteria are growing slowly

Trentay 17:55, 12 August 2009 (UTC)

Tasks

Maria and Afton

  • Miniprep more pSB1C3 p1010
  • Take Spectrophotometry measurements for J06702, pSB1C3, 101, 105, 106, 107
  • Digest promoters 101, 105, 106, 107 along with pSB1C3, J06702
  • Run a gel on the digests
    • Everything fit on the gel except for 106 and 107

Trentay 17:55, 12 August 2009 (UTC)

Wetlab

Kevin and Adam

  • I don't think that the no growth constructs were not successful. The last ligation reaction cleaned out the PCR product for D1-9. Will perform another PCR reaction today so the ligation can be done again.
    • Purified PCR product did not show up on gel. I will redo reaction tomorrow. Bussingkm 22:26, 12 August 2009 (UTC)


Maria and Afton

  • Miniprep was done
  • Spectrophotometry was done
  • Digest was done
  • Gel was run

Trentay 19:44, 12 August 2009 (UTC)

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