Virginia Commonwealth/8 July 2009
From 2009.igem.org
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*Digestion was successful and DNA from both plasmids (pSB4C5,pSB1C3) appeared on the gel after the electrophoresis test | *Digestion was successful and DNA from both plasmids (pSB4C5,pSB1C3) appeared on the gel after the electrophoresis test | ||
**Possibly pSB3K3 in the iGem stock is a bad part | **Possibly pSB3K3 in the iGem stock is a bad part | ||
+ | * Gel Electrophoresis Result | ||
+ | [[Image: 2009-07-08_digest04(psb1c3_and_psb4C5).jpg|350px|none|thumb|from left to right: pSB1C3,1kB ladder,pSB4C5]] | ||
[[User:Trentay|Trentay]] 18:50, 8 July 2009 (UTC) | [[User:Trentay|Trentay]] 18:50, 8 July 2009 (UTC) | ||
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Revision as of 16:55, 13 August 2009
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Contents |
Wednesday 8 July 2009
Results
Afton and Maria
- Digestion was successful and DNA from both plasmids (pSB4C5,pSB1C3) appeared on the gel after the electrophoresis test
- Possibly pSB3K3 in the iGem stock is a bad part
- Gel Electrophoresis Result
Trentay 18:50, 8 July 2009 (UTC)
Tasks
Afton and Maria
- Digestion of backbones: pSB1C3, pSB4C5
- Spectrometry of backbone miniprepped stocks
- Transform promoter 106, RET mCherry when cuvettes arrive
Trentay 16:49, 8 July 2009 (UTC)
Wetlab
Afton and Maria
- Digestion
- Agarose Gel preparation/Electrophoresis test
- Parts (J06702,J23106) were taken from iGem wells, electrotransformed, and plated.
Trentay 21:26, 8 July 2009 (UTC)