Team:Newcastle/Project/Labwork/OurProtocols/Making Agar Plates
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- | =Preparing the agar powder= | + | =Preparing the LB+agar powder= |
Mix the contents below to prepare the agar powder required to make 10L of agar solution | Mix the contents below to prepare the agar powder required to make 10L of agar solution | ||
*100gr tryptoptan | *100gr tryptoptan | ||
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Mix the content very well before using the mixed powder | Mix the content very well before using the mixed powder | ||
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+ | =Preparing the agar solution= | ||
+ | To prepare 1L of LB+agar solution | ||
+ | *Put 500 ml of distilled water to a tube | ||
+ | *Put 400ml of distilled water to a tube and 35 gr of LB+agar powder. Top the tube up to 500ml with distilled water | ||
+ | *Add 10ul of antifoam to LB+agar tube | ||
+ | *Put the tubes for autoclaving for half an hour | ||
+ | *Put the tube with LB+agar into 55C-60C water bath. | ||
+ | *Put the water tube into the sink to cool it down. | ||
+ | *If necessary, add the antibiotics to LB+agar tube after the tube cools down. | ||
+ | *Mix both tubes throughly | ||
+ | *Pour the plates using roughly 25ml of the solution for each Petri dish using the aseptic technique | ||
+ | |||
{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} |
Revision as of 12:27, 14 August 2009
Preparing the LB+agar powder
Mix the contents below to prepare the agar powder required to make 10L of agar solution
- 100gr tryptoptan
- 50gr yeast extract
- 50gr NaCl
- 150 gr agar
Mix the content very well before using the mixed powder
Preparing the agar solution
To prepare 1L of LB+agar solution
- Put 500 ml of distilled water to a tube
- Put 400ml of distilled water to a tube and 35 gr of LB+agar powder. Top the tube up to 500ml with distilled water
- Add 10ul of antifoam to LB+agar tube
- Put the tubes for autoclaving for half an hour
- Put the tube with LB+agar into 55C-60C water bath.
- Put the water tube into the sink to cool it down.
- If necessary, add the antibiotics to LB+agar tube after the tube cools down.
- Mix both tubes throughly
- Pour the plates using roughly 25ml of the solution for each Petri dish using the aseptic technique
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
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