"
Team:EPF-Lausanne/Last News
From 2009.igem.org
(Difference between revisions)
(→(08.08.09)) |
(→Keep track with what we did so far) |
||
| Line 40: | Line 40: | ||
==''(01.08.09)''== | ==''(01.08.09)''== | ||
| - | :This fourth week of wetlab we have done the following things | + | :This fourth week of wetlab we have done the following things: |
*Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick. | *Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick. | ||
*The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert | *The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert | ||
| Line 46: | Line 46: | ||
==''(24.07.09)''== | ==''(24.07.09)''== | ||
| - | :This third week of wetlab we have done the following things | + | :This third week of wetlab we have done the following things: |
*Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one. | *Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one. | ||
*Modeling part: Preliminar simulation was launched over the weekend. | *Modeling part: Preliminar simulation was launched over the weekend. | ||
| Line 52: | Line 52: | ||
==''(17.07.09)''== | ==''(17.07.09)''== | ||
| - | :This second week of wetlab we have done the following things | + | :This second week of wetlab we have done the following things: |
*Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one. | *Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one. | ||
*Modeling part: Files required to launch simulations were created and analyzed. | *Modeling part: Files required to launch simulations were created and analyzed. | ||
| Line 58: | Line 58: | ||
==''(12.07.09)''== | ==''(12.07.09)''== | ||
| - | :This first week of wetlab we have done the following things | + | :This first week of wetlab we have done the following things: |
*Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli | *Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli | ||
*Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.) | *Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.) | ||
Revision as of 14:59, 14 August 2009
Contents |
Keep track with what we did so far
(15.08.09)
- This sixth week of wetlab we have done the following things:
- Protocol of ligation was refined.
- !!! Inducible LOVTAP and Read out biobrick were created.!!! The system is completed.
- They need to be sequenced, characterized and submitted to the registery.
(08.08.09)
- This fifth week of wetlab we have done the following things:
- Problems in ligations.
(01.08.09)
- This fourth week of wetlab we have done the following things:
- Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick.
- The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
- Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.
(24.07.09)
- This third week of wetlab we have done the following things:
- Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
- Modeling part: Preliminar simulation was launched over the weekend.
(17.07.09)
- This second week of wetlab we have done the following things:
- Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
- Modeling part: Files required to launch simulations were created and analyzed.
(12.07.09)
- This first week of wetlab we have done the following things:
- Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
- Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
- Ordered and received the primers needed for the PCR of LovTAP
- Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
- Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
- Fused the two BioBricks "LacI" and "RBS"
- Digested the LovTAP PCR products and RBS part
