Team:Newcastle/Labwork/17 August 2009

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In our last lab session (14/08/09) starch agar plates were made and once this task had been completed, ''Bacillus subtilis'' was entered onto the plate. This was done by firstly drawing a grid of 46 squares on the base of the agar plate and then marking each square with the bacteria. Into square 1, untransformed wild type ''Bacillus subtilis'' was added and into squares 2-46, ''Bacillus subtilis'' transformed with ''gfp-rrnb'' was added. These plates were then grown overnight.  
In our last lab session (14/08/09) starch agar plates were made and once this task had been completed, ''Bacillus subtilis'' was entered onto the plate. This was done by firstly drawing a grid of 46 squares on the base of the agar plate and then marking each square with the bacteria. Into square 1, untransformed wild type ''Bacillus subtilis'' was added and into squares 2-46, ''Bacillus subtilis'' transformed with ''gfp-rrnb'' was added. These plates were then grown overnight.  
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The reason for this exercise is to see whether the bacteria that have survived the chloramphenicol treatment really have been given this property by taking up ''gfp-rrnb''. If the bacteria can't break down the starch which surrounds them on these plates then they have surely taken up the plasmid vector; if they can break down the starch then they might have received the resistance by other means. It may also mean that the vector may have integrated into another area in the ''Bacillus'''s chromosome. '''Today we shall be adding iodine to the plates so that we can assess whether the transformed ''B. subtilis'' can breakdown starch.'''
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The reason for this exercise is to see whether the bacteria that have survived the chloramphenicol treatment really have been given this property by taking up ''gfp-rrnb''. If the bacteria can't break down the starch which surrounds them on these plates then they have surely taken up the plasmid vector; if they can break down the starch then they might have received the resistance by other means. It may also mean that the vector may have integrated into another area in the ''Bacillus'''s chromosome. '''Today we shall be adding iodine to the plates so that we can assess whether the transformed ''B. subtilis'' can break down starch.'''
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Revision as of 23:13, 17 August 2009

Contents

Lab 17/08/09

Sporulation Tuning/ Chassis Team

Summary

Today, we plan to repeat the experiment which we did on the Wednesday,12th of August, which is the recovery of the cwlD spores.

We plan to follow the protocol for Method A again, however, this time, keeping in mind to add 40ul of our stock lysozyme instead of just 4ul.

We will carry out the usual treatment of the spores with lysozyme, buffer solution and L-alanine. However, we will also attempt one extra "treatment", which is adding buffer solution to the spores and carrying out the procedure as usual, and lastly adding L-alanine. What we are trying to achieve here, is how great an effect lysozyme has on the spores.

Metal Sensor Team

Introduction and Summary

In our last lab session (14/08/09) starch agar plates were made and once this task had been completed, Bacillus subtilis was entered onto the plate. This was done by firstly drawing a grid of 46 squares on the base of the agar plate and then marking each square with the bacteria. Into square 1, untransformed wild type Bacillus subtilis was added and into squares 2-46, Bacillus subtilis transformed with gfp-rrnb was added. These plates were then grown overnight.

The reason for this exercise is to see whether the bacteria that have survived the chloramphenicol treatment really have been given this property by taking up gfp-rrnb. If the bacteria can't break down the starch which surrounds them on these plates then they have surely taken up the plasmid vector; if they can break down the starch then they might have received the resistance by other means. It may also mean that the vector may have integrated into another area in the Bacillus's chromosome. Today we shall be adding iodine to the plates so that we can assess whether the transformed B. subtilis can break down starch.



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