Team:Newcastle/Metalsensing
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- | =Metal | + | =Metal Sensing= |
- | ==Introduction== | + | ==Introduction==] |
+ | If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating. | ||
- | + | There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA. | |
+ | By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation. | ||
- | + | Plan B allow us to meet a Gold metal criteria: we would improve BBa_J33206 (ArsR) BioBrick. | |
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==Modelling== | ==Modelling== | ||
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==BioBrick constructs== | ==BioBrick constructs== | ||
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==Lab Work Strategies== | ==Lab Work Strategies== |
Revision as of 21:14, 19 August 2009
Metal Sensing
==Introduction==] If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating.
There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA. By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation.
Plan B allow us to meet a Gold metal criteria: we would improve BBa_J33206 (ArsR) BioBrick.
Modelling
BioBrick constructs
Lab Work Strategies
Other Presentations and Diagrams
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]