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Team:Newcastle/Labwork/18 August 2009
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==<u>Sporulation Tuning/ Chassis Team</u>== | ==<u>Sporulation Tuning/ Chassis Team</u>== | ||
===Summary=== | ===Summary=== | ||
| - | [[Image:Newcastle 18 August BSub fume.jpg|thumb|The single colonies in the fume cabinet.] | + | [[Image:Newcastle 18 August BSub fume.jpg|thumb|The single colonies in the fume cabinet.]] |
| - | [[Image:Newcastle 18 August BSub grown single.jpg|thumb|The single colonies.] | + | [[Image:Newcastle 18 August BSub grown single.jpg|thumb|The single colonies.]] |
| - | [[Image:Newcastle 18 August BSub in fume 1.jpg|thumb|The single colonies in the fume cabinet.] | + | [[Image:Newcastle 18 August BSub in fume 1.jpg|thumb|The single colonies in the fume cabinet.]] |
| - | [[Image:Newcastle 18 August BSub in fume 2.jpg|thumb|The single colonies in the fume cabinet.] | + | [[Image:Newcastle 18 August BSub in fume 2.jpg|thumb|The single colonies in the fume cabinet.]] |
| - | [[Image:Newcastle 18 August BSub in fume.jpg|thumb|The single colonies in the fume cabinet.] | + | [[Image:Newcastle 18 August BSub in fume.jpg|thumb|The single colonies in the fume cabinet.]] |
[[Image:Newcastle 18 August cwlD 101 and 102.jpg|thumb|cwlD plates 10<sup>-1</sup> and 10<sup>-2</sup>.]] | [[Image:Newcastle 18 August cwlD 101 and 102.jpg|thumb|cwlD plates 10<sup>-1</sup> and 10<sup>-2</sup>.]] | ||
[[Image:Newcastle 18 August cwlD 103 and 104.jpg|thumb|cwlD plates 10<sup>-3</sup> and 10<sup>-4</sup>.]] | [[Image:Newcastle 18 August cwlD 103 and 104.jpg|thumb|cwlD plates 10<sup>-3</sup> and 10<sup>-4</sup>.]] | ||
Revision as of 22:37, 19 August 2009
Project
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Lab 18/08/09
Sporulation Tuning/ Chassis Team
Summary
Today, the moment of truth, the recovery of spores cwlD seemed to have worked judging from the results. A big sigh of relief from our team. Since it has been confirmed that the problem was that insufficient lysozyme stock solution was added, and that the current amount of lysozyme stock solution now works, we proceeded with the treatment for the recovery of spores sleB and cwlJ. The only and main difference is that the spores after treatment were plated out on plates with Kan and Em antibiotic.
We also did the iodine test which came back with relatively good results.
Lastly, we made up a new batch of LB solution for common use.
Results
Recovery of spores sleB and cwlJ
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
