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Team:Cambridge/Notebook/Week7
From 2009.igem.org
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PCR done of the origonal MelA and the MelA inbtermediate to biobrick (without the second PstI site). This was too create enough DNA for a resitriction digest to be carried out to check that the previous weeks PCRs had been sucessful. After PCR, the products were run on a gel and extracted. | PCR done of the origonal MelA and the MelA inbtermediate to biobrick (without the second PstI site). This was too create enough DNA for a resitriction digest to be carried out to check that the previous weeks PCRs had been sucessful. After PCR, the products were run on a gel and extracted. | ||
| - | We also tested the Primer C again, as it didn't work last time. Three PCR's were done | + | We also tested the Primer C again, as it didn't work last time. Three PCR's were done: |
| + | *Primer F and Primer C used last time | ||
| + | *Primer F and a new aliquot of Primer C | ||
| + | *Primer F and revortexed and re-aliquoted primer C from the origonal vial | ||
===Dry Work=== | ===Dry Work=== | ||
Revision as of 15:08, 24 August 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Week 7
Monday
Wet Work
Amplification
Colony PCR of 8 colonies picked from 391 in pSB3K3 transformants and 8 colonies picked from 374 in pSB3K3 transformants, overnight cultures of each of those colonies as well.
MelA BioBrick
PCR done of the origonal MelA and the MelA inbtermediate to biobrick (without the second PstI site). This was too create enough DNA for a resitriction digest to be carried out to check that the previous weeks PCRs had been sucessful. After PCR, the products were run on a gel and extracted.
We also tested the Primer C again, as it didn't work last time. Three PCR's were done:
- Primer F and Primer C used last time
- Primer F and a new aliquot of Primer C
- Primer F and revortexed and re-aliquoted primer C from the origonal vial
Dry Work
We found the phzM and phzS pigments in the registry!
- BBa_I723024 (phzM)
- BBa_I723025 (phzS)
This means we can now start working on these pigments, connecting them to processing and logic systems.
