Team:Newcastle/Project/Labwork/OurProtocols/PCR Products
From 2009.igem.org
(Difference between revisions)
Line 9: | Line 9: | ||
*Digest the PCR product | *Digest the PCR product | ||
Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration | Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration | ||
+ | |||
+ | Make a total solution of 70ul using the followings. (Check the restriction sites and replace EcoRI and SpeI as you need) | ||
+ | **50ul of the PCR product | ||
+ | **3ul of EcoRI | ||
+ | **3ul of SpeI | ||
+ | **7ul of 10X buffer | ||
+ | **Use water to make the final volume 70ul | ||
{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} |
Revision as of 14:26, 1 September 2009
- Use PCR Clean-up kit to clear the PCR product
This should result in 50ul of final product
- Measure the concentration of the product
This step will will be useful to determine how much of the product to use for the ligation
- Digest the PCR product
Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration
Make a total solution of 70ul using the followings. (Check the restriction sites and replace EcoRI and SpeI as you need)
- 50ul of the PCR product
- 3ul of EcoRI
- 3ul of SpeI
- 7ul of 10X buffer
- Use water to make the final volume 70ul
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]