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Team:Groningen/Parts
From 2009.igem.org
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|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.21 | |plate 3, pos.21 | ||
| - | |EcoRI/PstI + Sequenced | + | |EcoRI/PstI + {{done|Sequenced Okay}} |
|- | |- | ||
|Const. Pormoter + GVP (no.2) | |Const. Pormoter + GVP (no.2) | ||
| Line 175: | Line 175: | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.22 | |plate 3, pos.22 | ||
| - | |EcoRI/PstI + Sequenced | + | |EcoRI/PstI + {{done|Sequenced Okay}} |
|- | |- | ||
|Const. Pormoter + GVP | |Const. Pormoter + GVP | ||
| Line 184: | Line 184: | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.23 | |plate 3, pos.23 | ||
| - | |EcoRI/PstI + Sequenced | + | |EcoRI/PstI + {{done|Sequenced Okay}} |
|- | |- | ||
|Const. Pormoter + GVP (no.2) | |Const. Pormoter + GVP (no.2) | ||
| Line 193: | Line 193: | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.24 | |plate 3, pos.24 | ||
| - | |EcoRI/PstI + Sequenced | + | |EcoRI/PstI + {{done|Sequenced Okay}} |
|- | |- | ||
|HmtA (1.1) | |HmtA (1.1) | ||
| Line 220: | Line 220: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.37 | |plate 3, pos.37 | ||
| - | |Restriction(+) + Sequenced | + | |Restriction(+) + {{done|Sequenced Okay}} |
|- | |- | ||
|ZntR- + GVP in J61035 | |ZntR- + GVP in J61035 | ||
| Line 229: | Line 229: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.38 | |plate 3, pos.38 | ||
| - | |Restriction(+) + Sequenced (3 mutations) | + | |Restriction(+) + {{done|Sequenced (3 mutations)}} |
|- | |- | ||
|ArsR- + GVP in J61035 | |ArsR- + GVP in J61035 | ||
| Line 238: | Line 238: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.39 | |plate 3, pos.39 | ||
| - | |Restriction(+) + Sequenced | + | |Restriction(+) + {{done|Sequenced Okay}} |
|- | |- | ||
|ArsR- in J61002 | |ArsR- in J61002 | ||
| Line 247: | Line 247: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.40 | |plate 3, pos.40 | ||
| - | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced | + | |EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}} |
|- | |- | ||
|ZntR- in J61002 | |ZntR- in J61002 | ||
| Line 256: | Line 256: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.41 | |plate 3, pos.41 | ||
| - | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations) | + | |EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced (3 mutations)}} |
|- | |- | ||
|CueO- in J61002 | |CueO- in J61002 | ||
| Line 265: | Line 265: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.42 | |plate 3, pos.42 | ||
| - | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced | + | |EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}} |
|- | |- | ||
|ZntR+RBS in J61002 | |ZntR+RBS in J61002 | ||
| Line 274: | Line 274: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.43 | |plate 3, pos.43 | ||
| - | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced | + | |EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}} |
|- | |- | ||
|ArsR+RBS in J61002 | |ArsR+RBS in J61002 | ||
| Line 283: | Line 283: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.44 | |plate 3, pos.44 | ||
| - | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced | + | |EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}} |
|- | |- | ||
|CueO+RBS in J61002 | |CueO+RBS in J61002 | ||
| Line 292: | Line 292: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.45 | |plate 3, pos.45 | ||
| - | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced | + | |EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}} |
|- | |- | ||
|SmtA-GST in pGEX-3x | |SmtA-GST in pGEX-3x | ||
| Line 391: | Line 391: | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 59 | |plate 3, pos. 59 | ||
| - | |{{done|Sequence | + | |{{done|Sequence 2 deletions between EcoRI and XbaI sites}} |
|- | |- | ||
|High consitutive promoter(with RFP) | |High consitutive promoter(with RFP) | ||
Revision as of 14:15, 2 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| Biobricks
|
|---|
Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
- http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]
Part List -80
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well | Quality control |
| GVP | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.1 | ? |
| GVP (2nd batch) | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.2 | ? |
| pHigh in low copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 4 | PCR(?), restriction(+) | ||
| pMed in low copy nr vector | Medium consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 5 | PCR(?), restriction(+) | ||
| pLow in low copy nr vector | Low consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 6 | PCR(?), restriction(+) | ||
| pHigh in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 7 | PCR(?), restriction(+) | ||
| pMed in high copy nr vector | Medium consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 8 | PCR(?), restriction(+) | ||
| pLow in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 9 | PCR(?), restriction(+) | ||
| Promotor (low expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.10 | No |
| Promotor (medium expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.11 | No |
| Promotor (high expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.12 | No |
| Inducible promoter | Lactose inducible promoter nr1 | 200bp | Ampicillin | Plate 3, pos. 14 | PCR(?), restriction(+) | ||
| Inducible promoter | Lactose inducible promoter nr2 | 200bp | Ampicillin | Plate 3, pos. 15 | PCR(?), restriction(+) | ||
| RBS | Ribosomal Binding Site | [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | 12 | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | Ampicillin | plate 3, pos.19 | No |
| Terminator | Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011] | [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] | ~90 | [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] | Ampicillin/Kanamycin | plate 3, pos.20 | No |
| Const. Pormoter + GVP | Low constitutive promoter with GVP cluster | [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | ~6100 | [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] | Ampicillin/Chloramphenicol | plate 3, pos.21 | EcoRI/PstI + Sequenced Okay |
| Const. Pormoter + GVP (no.2) | Low constitutive promoter with GVP cluster | [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | ~6100 | [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] | Ampicillin/Chloramphenicol | plate 3, pos.22 | EcoRI/PstI + Sequenced Okay |
| Const. Pormoter + GVP | Medium constitutive promoter with GVP cluster | [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | ~6100 | [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] | Ampicillin/Chloramphenicol | plate 3, pos.23 | EcoRI/PstI + Sequenced Okay |
| Const. Pormoter + GVP (no.2) | Medium constitutive promoter with GVP cluster | [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | ~6100 | [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] | Ampicillin/Chloramphenicol | plate 3, pos.24 | EcoRI/PstI + Sequenced Okay |
| HmtA (1.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.28 | Restriction(+) |
| HmtA (2.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.29 | Restriction(+) |
| CueO- + GVP in J61035 | CueO promoter without RBS in front of the GVP cluster | None | 6149 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.37 | Restriction(+) + Sequenced Okay |
| ZntR- + GVP in J61035 | ZntR promoter without RBS in front of the GVP cluster | None | ~6150 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.38 | Restriction(+) + Sequenced (3 mutations) |
| ArsR- + GVP in J61035 | ArsR promoter without RBS in front of the GVP cluster | None | ~6150 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.39 | Restriction(+) + Sequenced Okay |
| ArsR- in J61002 | pArsR (promoter without RBS) | BBa_K190015 | 71 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.40 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay |
| ZntR- in J61002 | pZntR (promoter without RBS) | BBa_K190016 | 65 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.41 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations) |
| CueO- in J61002 | pCueO (promoter without RBS) | BBa_K190017 | 43 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.42 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay |
| ZntR+RBS in J61002 | pZntR (promoter with own RBS) | BBa_K190022 | 91 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.43 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay |
| ArsR+RBS in J61002 | pArsR (promoter with own RBS) | BBa_K190023 | 81 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.44 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay |
| CueO+RBS in J61002 | pCueO (promoter with own RBS) | BBa_K190024 | 71 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.45 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay |
| SmtA-GST in pGEX-3x | SmtA-GST fusion | no biobrick primers available | 1400bp | pGEX-3x (NEB) | Ampicillin | plate 3, pos. 46 | PCR, restriction |
| SmtA in pET29a | SmtA | 900bp | pET29a (NEB) | Kanamycin | plate 3, pos. 47 | PCR, restriction | |
| MymT in pGB68 | MymT | 215bp | Ampicillin | pTXB1 (NEB) | plate 3, pos. 48 | PCR, restriction | |
| GlpF and fMT in pMAL-MT | GlpF and fMT on a synthetic operon in the vector | and BBa_K1900?? (GlpF has no BioBrick nr yet) | fMT = 200bp, GlpF = 900bp? | Ampicillin | pMALc2x (NEB) | plate 3, pos. 49 | PCR, restriction |
| MBP-ArsR | MBP-ArsR Fusion protein | 1500 bp | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 53 | Restriction analysis] & PCR | |
| pNL29 | GVP Gene cluster | 6000 bp | pBluescriptIIKS | Ampicillin | plate 3, pos. 54 | Buoyancy phenotype | |
| pLac-fMT | Metallothionein with inducible promotor | Part or Accession # | Base Pairs (bp) | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 55 | Quality control |
| pLow-fMT #4 | Metallothionein with low constitutive promotor | Part or Accession # | Base Pairs (bp) | pSB1A2 | Ampicillin/chloramphenicol | plate 3, pos. 56 | Quality control |
| pLow-fMT #3 | Metallothionein with low constitutive promotor | Part or Accession # | Base Pairs (bp) | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 57 | Quality control |
| SmtA | Metallothionein | Part or Accession # | Base Pairs (bp) | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 58 | Quality control |
| fMT #6 | Metallothionein for As3+ | Part or Accession # | Base Pairs (bp) | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 59 | Sequence 2 deletions between EcoRI and XbaI sites |
| High consitutive promoter(with RFP) | Constitutive promoter | Bba_J23100 | Base Pairs (bp) | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 75 | Quality control |
| Medium consitutive promoter(with RFP) | Constitutive promoter | Bba_J23106 | Base Pairs (bp) | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 76 | Quality control |
| Low consitutive promoter(with RFP) | Constitutive promoter | Bba_J23109 | Base Pairs (bp) | pSB1AC3 | Ampicillin/chloramphenicol | plate 3, pos. 77 | Quality control |
| High consitutive promoter(with RFP) | Constitutive promoter | Bba_J23100 | Base Pairs (bp) | pSB3K3 | Kanamycin | plate 3, pos. 78 | Quality control |
| Medium consitutive promoter(with RFP) | Constitutive promoter | Bba_J23106 | Base Pairs (bp) | pSB3K3 | Kanamycin | plate 3, pos. 79 | Quality control |
| Low consitutive promoter(with RFP) | Constitutive promoter | Bba_J23109 | Base Pairs (bp) | pSB3K3 | Kanamycin | plate 3, pos. 80 | Quality control |
| Reference constitutive promoter) | Constitutive promoter | ? | Base Pairs (bp) | ? | ? | plate 3, pos. 81 | Quality control |
