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Team:Groningen/Parts
From 2009.igem.org
(Difference between revisions)
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| Line 26: | Line 26: | ||
|GVP | |GVP | ||
|Gas Vesicle Proteins | |Gas Vesicle Proteins | ||
| - | | | + | |<partinfo>BBa_I750016</partinfo> |
|6064 | |6064 | ||
| - | | | + | |<partinfo>BBa_J61035</partinfo> |
|Ampicillin/Gentamycin | |Ampicillin/Gentamycin | ||
|plate 3, pos.1 | |plate 3, pos.1 | ||
| Line 35: | Line 35: | ||
|GVP (2nd batch) | |GVP (2nd batch) | ||
|Gas Vesicle Proteins | |Gas Vesicle Proteins | ||
| - | | | + | |<partinfo>BBa_I750016</partinfo> |
|6064 | |6064 | ||
| - | | | + | |<partinfo>BBa_J61035</partinfo> |
|Ampicillin/Gentamycin | |Ampicillin/Gentamycin | ||
|plate 3, pos.2 | |plate 3, pos.2 | ||
| Line 98: | Line 98: | ||
|Promotor (low expression) | |Promotor (low expression) | ||
|Constitutive promotor | |Constitutive promotor | ||
| - | | | + | |<partinfo>BBa_J23109</partinfo> |
|35 | |35 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.10 | |plate 3, pos.10 | ||
| Line 107: | Line 107: | ||
|Promotor (medium expression) | |Promotor (medium expression) | ||
|Constitutive promotor | |Constitutive promotor | ||
| - | | | + | |<partinfo>BBa_J23106</partinfo> |
|35 | |35 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.11 | |plate 3, pos.11 | ||
| Line 116: | Line 116: | ||
|Promotor (high expression) | |Promotor (high expression) | ||
|Constitutive promotor | |Constitutive promotor | ||
| - | | | + | |<partinfo>BBa_J23100</partinfo> |
|35 | |35 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.12 | |plate 3, pos.12 | ||
| Line 143: | Line 143: | ||
|RBS | |RBS | ||
|Ribosomal Binding Site | |Ribosomal Binding Site | ||
| - | | | + | |<partinfo>BBa_B0034</partinfo> |
|12 | |12 | ||
| - | | | + | |<partinfo>pSB1A2</partinfo> |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.19 | |plate 3, pos.19 | ||
| Line 151: | Line 151: | ||
|- | |- | ||
|Terminator | |Terminator | ||
| - | |Double terminator consisting of | + | |Double terminator consisting of <partinfo>BBa_B0012</partinfo> and <partinfo>BBa_B0011</partinfo>] |
| - | | | + | |<partinfo>BBa_B0014</partinfo> |
|~90 | |~90 | ||
| - | | | + | |<partinfo>pSB1AK3</partinfo> |
|Ampicillin/Kanamycin | |Ampicillin/Kanamycin | ||
|plate 3, pos.20 | |plate 3, pos.20 | ||
| Line 161: | Line 161: | ||
|Const. Pormoter + GVP | |Const. Pormoter + GVP | ||
|Low constitutive promoter with GVP cluster | |Low constitutive promoter with GVP cluster | ||
| - | | | + | |<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo> |
|~6100 | |~6100 | ||
| - | | | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.21 | |plate 3, pos.21 | ||
| - | | | + | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}} |
|- | |- | ||
|Const. Pormoter + GVP (no.2) | |Const. Pormoter + GVP (no.2) | ||
|Low constitutive promoter with GVP cluster | |Low constitutive promoter with GVP cluster | ||
| - | | | + | |<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo> |
|~6100 | |~6100 | ||
| - | | | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.22 | |plate 3, pos.22 | ||
| - | | | + | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}} |
|- | |- | ||
|Const. Pormoter + GVP | |Const. Pormoter + GVP | ||
|Medium constitutive promoter with GVP cluster | |Medium constitutive promoter with GVP cluster | ||
| - | | | + | |<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo> |
|~6100 | |~6100 | ||
| - | | | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.23 | |plate 3, pos.23 | ||
| - | | | + | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}} |
|- | |- | ||
|Const. Pormoter + GVP (no.2) | |Const. Pormoter + GVP (no.2) | ||
|Medium constitutive promoter with GVP cluster | |Medium constitutive promoter with GVP cluster | ||
| - | | | + | |<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo> |
|~6100 | |~6100 | ||
| - | | | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|plate 3, pos.24 | |plate 3, pos.24 | ||
| - | | | + | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}} |
|- | |- | ||
|HmtA (1.1) | |HmtA (1.1) | ||
| Line 217: | Line 217: | ||
|None | |None | ||
|6149 | |6149 | ||
| - | | | + | |<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.37 | |plate 3, pos.37 | ||
| Line 226: | Line 226: | ||
|None | |None | ||
|~6150 | |~6150 | ||
| - | | | + | |<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.38 | |plate 3, pos.38 | ||
| Line 235: | Line 235: | ||
|None | |None | ||
|~6150 | |~6150 | ||
| - | | | + | |<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.39 | |plate 3, pos.39 | ||
| Line 242: | Line 242: | ||
|ArsR- in J61002 | |ArsR- in J61002 | ||
|pArsR (promoter without RBS) | |pArsR (promoter without RBS) | ||
| - | |BBa_K190015 | + | |<partinfo>BBa_K190015</partinfo> |
|71 | |71 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.40 | |plate 3, pos.40 | ||
| - | | | + | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}} |
|- | |- | ||
|ZntR- in J61002 | |ZntR- in J61002 | ||
|pZntR (promoter without RBS) | |pZntR (promoter without RBS) | ||
| - | |BBa_K190016 | + | |<partinfo>BBa_K190016</partinfo> |
|65 | |65 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.41 | |plate 3, pos.41 | ||
| - | | | + | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced (3 mutations)}} |
|- | |- | ||
|CueO- in J61002 | |CueO- in J61002 | ||
|pCueO (promoter without RBS) | |pCueO (promoter without RBS) | ||
| - | |BBa_K190017 | + | |<partinfo>BBa_K190017</partinfo> |
|43 | |43 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.42 | |plate 3, pos.42 | ||
| - | | | + | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}} |
|- | |- | ||
|ZntR+RBS in J61002 | |ZntR+RBS in J61002 | ||
|pZntR (promoter with own RBS) | |pZntR (promoter with own RBS) | ||
| - | |BBa_K190022 | + | |<partinfo>BBa_K190022</partinfo> |
|91 | |91 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.43 | |plate 3, pos.43 | ||
| - | | | + | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}} |
|- | |- | ||
|ArsR+RBS in J61002 | |ArsR+RBS in J61002 | ||
|pArsR (promoter with own RBS) | |pArsR (promoter with own RBS) | ||
| - | |BBa_K190023 | + | |<partinfo>BBa_K190023</partinfo> |
|81 | |81 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.44 | |plate 3, pos.44 | ||
| - | | | + | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}} |
|- | |- | ||
|CueO+RBS in J61002 | |CueO+RBS in J61002 | ||
|pCueO (promoter with own RBS) | |pCueO (promoter with own RBS) | ||
| - | |BBa_K190024 | + | |<partinfo>BBa_K190024</partinfo> |
|71 | |71 | ||
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.45 | |plate 3, pos.45 | ||
| - | | | + | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}} |
|- | |- | ||
|SmtA-GST in pGEX-3x | |SmtA-GST in pGEX-3x | ||
| Line 323: | Line 323: | ||
|GlpF and fMT in pMAL-MT | |GlpF and fMT in pMAL-MT | ||
|GlpF and fMT on a synthetic operon in the vector | |GlpF and fMT on a synthetic operon in the vector | ||
| - | |<partinfo>BBa_K190019</partinfo> and | + | |<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo> |
|fMT = 200bp, GlpF = 900bp? | |fMT = 200bp, GlpF = 900bp? | ||
|Ampicillin | |Ampicillin | ||
| Line 332: | Line 332: | ||
|MBP-ArsR | |MBP-ArsR | ||
|MBP-ArsR Fusion protein | |MBP-ArsR Fusion protein | ||
| - | | | + | |<partinfo>BBa_K190027</partinfo> |
| - | | | + | |15180 bp |
| - | |pSB1AC3 | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 53 | |plate 3, pos. 53 | ||
| Line 350: | Line 350: | ||
|pLac-fMT | |pLac-fMT | ||
|Metallothionein with inducible promotor | |Metallothionein with inducible promotor | ||
| - | | | + | |<partinfo>BBa_K190032</partinfo> |
| - | | | + | |430 bp |
| - | | | + | |<partinfo>pSB1A2</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 55 | |plate 3, pos. 55 | ||
| Line 359: | Line 359: | ||
|pLow-fMT #4 | |pLow-fMT #4 | ||
|Metallothionein with low constitutive promotor | |Metallothionein with low constitutive promotor | ||
| - | | | + | |<partinfo>BBa_K190031</partinfo> |
| - | | | + | |265 bp |
| - | | | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 56 | |plate 3, pos. 56 | ||
| Line 368: | Line 368: | ||
|pLow-fMT #3 | |pLow-fMT #3 | ||
|Metallothionein with low constitutive promotor | |Metallothionein with low constitutive promotor | ||
| - | | | + | |<partinfo>BBa_K190031</partinfo> |
| - | | | + | |265 bp |
| - | |pSB1AC3 | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 57 | |plate 3, pos. 57 | ||
| Line 377: | Line 377: | ||
|SmtA | |SmtA | ||
|Metallothionein | |Metallothionein | ||
| - | | | + | |<partinfo>BBa_K190021</partinfo> |
| - | | | + | |678 bp |
| - | |pSB1AC3 | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 58 | |plate 3, pos. 58 | ||
| Line 386: | Line 386: | ||
|fMT #6 | |fMT #6 | ||
|Metallothionein for As<sup>3+</sup> | |Metallothionein for As<sup>3+</sup> | ||
| - | | | + | |<partinfo>BBa_K190019</partinfo> |
| - | | | + | |222 bp |
| - | |pSB1AC3 | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 59 | |plate 3, pos. 59 | ||
| - | |{{ | + | |{{todo|Sequence 2 deletions between <i>Eco</i>RI and <i>Xba</i>I sites}} |
| + | |- | ||
| + | |fMT #4 | ||
| + | |Metallothionein for As<sup>3+</sup> | ||
| + | |<partinfo>BBa_K190019</partinfo> | ||
| + | | 222 bp | ||
| + | |<partinfo>pSB1AC3</partinfo> | ||
| + | |Ampicillin/chloramphenicol | ||
| + | |{{todo|plate 3, pos. 60}} | ||
| + | |{{done|Sequence Okay!}} | ||
|- | |- | ||
|High consitutive promoter(with RFP) | |High consitutive promoter(with RFP) | ||
|Constitutive promoter | |Constitutive promoter | ||
| - | |Bba_J23100 | + | |<partinfo>Bba_J23100</partinfo> |
|{{todo|Base Pairs (bp)}} | |{{todo|Base Pairs (bp)}} | ||
| - | |pSB1AC3 | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 75 | |plate 3, pos. 75 | ||
| Line 404: | Line 413: | ||
|Medium consitutive promoter(with RFP) | |Medium consitutive promoter(with RFP) | ||
|Constitutive promoter | |Constitutive promoter | ||
| - | |Bba_J23106 | + | |<partinfo>Bba_J23106</partinfo> |
|{{todo|Base Pairs (bp)}} | |{{todo|Base Pairs (bp)}} | ||
| - | |pSB1AC3 | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 76 | |plate 3, pos. 76 | ||
| Line 413: | Line 422: | ||
|Low consitutive promoter(with RFP) | |Low consitutive promoter(with RFP) | ||
|Constitutive promoter | |Constitutive promoter | ||
| - | |Bba_J23109 | + | |<partinfo>Bba_J23109</partinfo> |
|{{todo|Base Pairs (bp)}} | |{{todo|Base Pairs (bp)}} | ||
| - | |pSB1AC3 | + | |<partinfo>pSB1AC3</partinfo> |
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
|plate 3, pos. 77 | |plate 3, pos. 77 | ||
| Line 422: | Line 431: | ||
|High consitutive promoter(with RFP) | |High consitutive promoter(with RFP) | ||
|Constitutive promoter | |Constitutive promoter | ||
| - | |Bba_J23100 | + | |<partinfo>Bba_J23100</partinfo> |
|{{todo|Base Pairs (bp)}} | |{{todo|Base Pairs (bp)}} | ||
| - | |pSB3K3 | + | |<partinfo>pSB3K3</partinfo> |
|Kanamycin | |Kanamycin | ||
|plate 3, pos. 78 | |plate 3, pos. 78 | ||
| Line 431: | Line 440: | ||
|Medium consitutive promoter(with RFP) | |Medium consitutive promoter(with RFP) | ||
|Constitutive promoter | |Constitutive promoter | ||
| - | |Bba_J23106 | + | |<partinfo>Bba_J23106</partinfo> |
|{{todo|Base Pairs (bp)}} | |{{todo|Base Pairs (bp)}} | ||
| - | |pSB3K3 | + | |<partinfo>pSB3K3</partinfo> |
|Kanamycin | |Kanamycin | ||
|plate 3, pos. 79 | |plate 3, pos. 79 | ||
| Line 440: | Line 449: | ||
|Low consitutive promoter(with RFP) | |Low consitutive promoter(with RFP) | ||
|Constitutive promoter | |Constitutive promoter | ||
| - | |Bba_J23109 | + | |<partinfo>Bba_J23109</partinfo> |
|{{todo|Base Pairs (bp)}} | |{{todo|Base Pairs (bp)}} | ||
| - | |pSB3K3 | + | |<partinfo>pSB3K3</partinfo> |
|Kanamycin | |Kanamycin | ||
|plate 3, pos. 80 | |plate 3, pos. 80 | ||
Revision as of 08:10, 4 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| Biobricks
|
|---|
Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
- http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]
Part List -80
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well | Quality control |
| GVP | Gas Vesicle Proteins | 6064 | Ampicillin/Gentamycin | plate 3, pos.1 | ? | ||
| GVP (2nd batch) | Gas Vesicle Proteins | 6064 | Ampicillin/Gentamycin | plate 3, pos.2 | ? | ||
| pHigh in low copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 4 | PCR(?), restriction(+) | ||
| pMed in low copy nr vector | Medium consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 5 | PCR(?), restriction(+) | ||
| pLow in low copy nr vector | Low consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 6 | PCR(?), restriction(+) | ||
| pHigh in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 7 | PCR(?), restriction(+) | ||
| pMed in high copy nr vector | Medium consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 8 | PCR(?), restriction(+) | ||
| pLow in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 9 | PCR(?), restriction(+) | ||
| Promotor (low expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.10 | No | ||
| Promotor (medium expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.11 | No | ||
| Promotor (high expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.12 | No | ||
| Inducible promoter | Lactose inducible promoter nr1 | 200bp | Ampicillin | Plate 3, pos. 14 | PCR(?), restriction(+) | ||
| Inducible promoter | Lactose inducible promoter nr2 | 200bp | Ampicillin | Plate 3, pos. 15 | PCR(?), restriction(+) | ||
| RBS | Ribosomal Binding Site | 12 | Ampicillin | plate 3, pos.19 | No | ||
| Terminator | Double terminator consisting of and ] | ~90 | Ampicillin/Kanamycin | plate 3, pos.20 | No | ||
| Const. Pormoter + GVP | Low constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.21 | EcoRI/PstI + Sequenced Okay | |
| Const. Pormoter + GVP (no.2) | Low constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.22 | EcoRI/PstI + Sequenced Okay | |
| Const. Pormoter + GVP | Medium constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.23 | EcoRI/PstI + Sequenced Okay | |
| Const. Pormoter + GVP (no.2) | Medium constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.24 | EcoRI/PstI + Sequenced Okay | |
| HmtA (1.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.28 | Restriction(+) |
| HmtA (2.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.29 | Restriction(+) |
| CueO- + GVP in J61035 | CueO promoter without RBS in front of the GVP cluster | None | 6149 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.37 | Restriction(+) + Sequenced Okay |
| ZntR- + GVP in J61035 | ZntR promoter without RBS in front of the GVP cluster | None | ~6150 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.38 | Restriction(+) + Sequenced (3 mutations) |
| ArsR- + GVP in J61035 | ArsR promoter without RBS in front of the GVP cluster | None | ~6150 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.39 | Restriction(+) + Sequenced Okay |
| ArsR- in J61002 | pArsR (promoter without RBS) | 71 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.40 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ZntR- in J61002 | pZntR (promoter without RBS) | 65 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.41 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations) | |
| CueO- in J61002 | pCueO (promoter without RBS) | 43 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.42 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ZntR+RBS in J61002 | pZntR (promoter with own RBS) | 91 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.43 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ArsR+RBS in J61002 | pArsR (promoter with own RBS) | 81 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.44 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| CueO+RBS in J61002 | pCueO (promoter with own RBS) | 71 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.45 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| SmtA-GST in pGEX-3x | SmtA-GST fusion | no biobrick primers available | 1400bp | pGEX-3x (NEB) | Ampicillin | plate 3, pos. 46 | PCR, restriction |
| SmtA in pET29a | SmtA | 900bp | pET29a (NEB) | Kanamycin | plate 3, pos. 47 | PCR, restriction | |
| MymT in pGB68 | MymT | 215bp | Ampicillin | pTXB1 (NEB) | plate 3, pos. 48 | PCR, restriction | |
| GlpF and fMT in pMAL-MT | GlpF and fMT on a synthetic operon in the vector | and | fMT = 200bp, GlpF = 900bp? | Ampicillin | pMALc2x (NEB) | plate 3, pos. 49 | PCR, restriction |
| MBP-ArsR | MBP-ArsR Fusion protein | 15180 bp | Ampicillin/chloramphenicol | plate 3, pos. 53 | Restriction analysis] & PCR | ||
| pNL29 | GVP Gene cluster | 6000 bp | pBluescriptIIKS | Ampicillin | plate 3, pos. 54 | Buoyancy phenotype | |
| pLac-fMT | Metallothionein with inducible promotor | 430 bp | Ampicillin/chloramphenicol | plate 3, pos. 55 | Quality control | ||
| pLow-fMT #4 | Metallothionein with low constitutive promotor | 265 bp | Ampicillin/chloramphenicol | plate 3, pos. 56 | Quality control | ||
| pLow-fMT #3 | Metallothionein with low constitutive promotor | 265 bp | Ampicillin/chloramphenicol | plate 3, pos. 57 | Quality control | ||
| SmtA | Metallothionein | 678 bp | Ampicillin/chloramphenicol | plate 3, pos. 58 | Quality control | ||
| fMT #6 | Metallothionein for As3+ | 222 bp | Ampicillin/chloramphenicol | plate 3, pos. 59 | Sequence 2 deletions between EcoRI and XbaI sites | ||
| fMT #4 | Metallothionein for As3+ | 222 bp | Ampicillin/chloramphenicol | plate 3, pos. 60 | Sequence Okay! | ||
| High consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 75 | Quality control | ||
| Medium consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 76 | Quality control | ||
| Low consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 77 | Quality control | ||
| High consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 78 | Quality control | ||
| Medium consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 79 | Quality control | ||
| Low consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 80 | Quality control | ||
| Reference constitutive promoter) | Constitutive promoter | ? | Base Pairs (bp) | ? | ? | plate 3, pos. 81 | Quality control |
