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Team:Groningen/Parts
From 2009.igem.org
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| Line 45: | Line 45: | ||
|High consitutive promoter | |High consitutive promoter | ||
|<partinfo>J23100</partinfo> | |<partinfo>J23100</partinfo> | ||
| - | | | + | |35 |
|<partinfo>pSB3K3</partinfo> | |<partinfo>pSB3K3</partinfo> | ||
|Kanamycin | |Kanamycin | ||
| Line 54: | Line 54: | ||
|Medium consitutive promoter | |Medium consitutive promoter | ||
|<partinfo>J23106</partinfo> | |<partinfo>J23106</partinfo> | ||
| - | | | + | |35 |
|<partinfo>pSB3K3</partinfo> | |<partinfo>pSB3K3</partinfo> | ||
|Kanamycin | |Kanamycin | ||
| Line 63: | Line 63: | ||
|Low consitutive promoter | |Low consitutive promoter | ||
|<partinfo>J23109</partinfo> | |<partinfo>J23109</partinfo> | ||
| - | | | + | |35 |
|<partinfo>pSB3K3</partinfo> | |<partinfo>pSB3K3</partinfo> | ||
|Kanamycin | |Kanamycin | ||
| Line 72: | Line 72: | ||
|High consitutive promoter | |High consitutive promoter | ||
|<partinfo>J23100</partinfo> | |<partinfo>J23100</partinfo> | ||
| - | | | + | |35 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
| Line 81: | Line 81: | ||
|Medium consitutive promoter | |Medium consitutive promoter | ||
|<partinfo>J23106</partinfo> | |<partinfo>J23106</partinfo> | ||
| - | | | + | |35 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
| Line 90: | Line 90: | ||
|High consitutive promoter | |High consitutive promoter | ||
|<partinfo>J23109</partinfo> | |<partinfo>J23109</partinfo> | ||
| - | | | + | |35 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
| Line 126: | Line 126: | ||
|Lactose inducible promoter nr1 | |Lactose inducible promoter nr1 | ||
|<partinfo>R0010</partinfo> | |<partinfo>R0010</partinfo> | ||
| - | | | + | |200 |
|<partinfo>pSB1A2</partinfo> | |<partinfo>pSB1A2</partinfo> | ||
|Ampicillin | |Ampicillin | ||
| Line 135: | Line 135: | ||
|Lactose inducible promoter nr2 | |Lactose inducible promoter nr2 | ||
|<partinfo>R0010</partinfo> | |<partinfo>R0010</partinfo> | ||
| - | | | + | |200 |
|<partinfo>pSB1A2</partinfo> | |<partinfo>pSB1A2</partinfo> | ||
|Ampicillin | |Ampicillin | ||
| Line 289: | Line 289: | ||
|<partinfo>BBa_K190024</partinfo> | |<partinfo>BBa_K190024</partinfo> | ||
|71 | |71 | ||
| - | | | + | |<partinfo>BBa_J61002</partinfo> (without the part between EcoRI and SpeI) |
|Ampicillin | |Ampicillin | ||
|plate 3, pos.45 | |plate 3, pos.45 | ||
| Line 297: | Line 297: | ||
|SmtA-GST fusion | |SmtA-GST fusion | ||
|no biobrick primers available | |no biobrick primers available | ||
| - | | | + | |1400 |
| - | |pGEX-3x | + | |[http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x] |
|Ampicillin | |Ampicillin | ||
|plate 3, pos. 46 | |plate 3, pos. 46 | ||
| Line 306: | Line 306: | ||
|SmtA | |SmtA | ||
|<partinfo>BBa_K190021</partinfo> | |<partinfo>BBa_K190021</partinfo> | ||
| - | | | + | |900 |
| - | |pET29a | + | |[http://www.merckbiosciences.co.uk/product/69871 pET29a] |
|Kanamycin | |Kanamycin | ||
|plate 3, pos. 47 | |plate 3, pos. 47 | ||
| Line 315: | Line 315: | ||
|MymT | |MymT | ||
|<partinfo>BBa_K190020</partinfo> | |<partinfo>BBa_K190020</partinfo> | ||
| - | | | + | |215 |
| + | |[http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB) | ||
|Ampicillin | |Ampicillin | ||
| - | |||
|plate 3, pos. 48 | |plate 3, pos. 48 | ||
|PCR, restriction | |PCR, restriction | ||
| Line 324: | Line 324: | ||
|GlpF and fMT on a synthetic operon in the vector | |GlpF and fMT on a synthetic operon in the vector | ||
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo> | |<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo> | ||
| - | |fMT = | + | |fMT = 200, GlpF = 900? |
| + | |[http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB) | ||
|Ampicillin | |Ampicillin | ||
| - | |||
|plate 3, pos. 49 | |plate 3, pos. 49 | ||
|PCR, restriction | |PCR, restriction | ||
| Line 333: | Line 333: | ||
|MBP-ArsR Fusion protein | |MBP-ArsR Fusion protein | ||
|<partinfo>BBa_K190027</partinfo> | |<partinfo>BBa_K190027</partinfo> | ||
| - | |15180 | + | |15180 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
| Line 342: | Line 342: | ||
|GVP Gene cluster | |GVP Gene cluster | ||
|{{todo|}} | |{{todo|}} | ||
| - | |6000 | + | |6000 |
| - | |pBluescriptIIKS | + | |[http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS] |
|{{todo| Ampicillin}} | |{{todo| Ampicillin}} | ||
|plate 3, pos. 54 | |plate 3, pos. 54 | ||
| Line 351: | Line 351: | ||
|Metallothionein with inducible promotor | |Metallothionein with inducible promotor | ||
|<partinfo>BBa_K190032</partinfo> | |<partinfo>BBa_K190032</partinfo> | ||
| - | |430 | + | |430 |
|<partinfo>pSB1A2</partinfo> | |<partinfo>pSB1A2</partinfo> | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
| Line 360: | Line 360: | ||
|Metallothionein with low constitutive promotor | |Metallothionein with low constitutive promotor | ||
|<partinfo>BBa_K190031</partinfo> | |<partinfo>BBa_K190031</partinfo> | ||
| - | |265 | + | |265 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
| Line 369: | Line 369: | ||
|Metallothionein with low constitutive promotor | |Metallothionein with low constitutive promotor | ||
|<partinfo>BBa_K190031</partinfo> | |<partinfo>BBa_K190031</partinfo> | ||
| - | |265 | + | |265 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
| Line 378: | Line 378: | ||
|Metallothionein | |Metallothionein | ||
|<partinfo>BBa_K190021</partinfo> | |<partinfo>BBa_K190021</partinfo> | ||
| - | |678 | + | |678 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
| Line 387: | Line 387: | ||
|Metallothionein for As<sup>3+</sup> | |Metallothionein for As<sup>3+</sup> | ||
|<partinfo>BBa_K190019</partinfo> | |<partinfo>BBa_K190019</partinfo> | ||
| - | |222 | + | |222 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
| Line 396: | Line 396: | ||
|Metallothionein for As<sup>3+</sup> | |Metallothionein for As<sup>3+</sup> | ||
|<partinfo>BBa_K190019</partinfo> | |<partinfo>BBa_K190019</partinfo> | ||
| - | | 222 | + | | 222 |
|<partinfo>pSB1AC3</partinfo> | |<partinfo>pSB1AC3</partinfo> | ||
|Ampicillin/chloramphenicol | |Ampicillin/chloramphenicol | ||
Revision as of 08:31, 4 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| Biobricks
|
|---|
Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
- http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]
Part List -80
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well | Quality control |
| GVP | Gas Vesicle Proteins | 6064 | Ampicillin/Gentamycin | plate 3, pos.1 | ? | ||
| GVP (2nd batch) | Gas Vesicle Proteins | 6064 | Ampicillin/Gentamycin | plate 3, pos.2 | ? | ||
| pHigh in low copy nr vector | High consitutive promoter | 35 | Kanamycin | Plate 3, pos. 4 | PCR(?), restriction(+) | ||
| pMed in low copy nr vector | Medium consitutive promoter | 35 | Kanamycin | Plate 3, pos. 5 | PCR(?), restriction(+) | ||
| pLow in low copy nr vector | Low consitutive promoter | 35 | Kanamycin | Plate 3, pos. 6 | PCR(?), restriction(+) | ||
| pHigh in high copy nr vector | High consitutive promoter | 35 | Ampicillin/Chloramphenicol | Plate 3, pos. 7 | PCR(?), restriction(+) | ||
| pMed in high copy nr vector | Medium consitutive promoter | 35 | Ampicillin/Chloramphenicol | Plate 3, pos. 8 | PCR(?), restriction(+) | ||
| pLow in high copy nr vector | High consitutive promoter | 35 | Ampicillin/Chloramphenicol | Plate 3, pos. 9 | PCR(?), restriction(+) | ||
| Promotor (low expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.10 | No | ||
| Promotor (medium expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.11 | No | ||
| Promotor (high expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.12 | No | ||
| Inducible promoter | Lactose inducible promoter nr1 | 200 | Ampicillin | Plate 3, pos. 14 | PCR(?), restriction(+) | ||
| Inducible promoter | Lactose inducible promoter nr2 | 200 | Ampicillin | Plate 3, pos. 15 | PCR(?), restriction(+) | ||
| RBS | Ribosomal Binding Site | 12 | Ampicillin | plate 3, pos.19 | No | ||
| Terminator | Double terminator consisting of and ] | ~90 | Ampicillin/Kanamycin | plate 3, pos.20 | No | ||
| Const. Pormoter + GVP | Low constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.21 | EcoRI/PstI + Sequenced Okay | |
| Const. Pormoter + GVP (no.2) | Low constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.22 | EcoRI/PstI + Sequenced Okay | |
| Const. Pormoter + GVP | Medium constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.23 | EcoRI/PstI + Sequenced Okay | |
| Const. Pormoter + GVP (no.2) | Medium constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.24 | EcoRI/PstI + Sequenced Okay | |
| HmtA (1.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.28 | Restriction(+) |
| HmtA (2.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.29 | Restriction(+) |
| CueO- + GVP in J61035 | CueO promoter without RBS in front of the GVP cluster | None | 6149 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.37 | Restriction(+) + Sequenced Okay |
| ZntR- + GVP in J61035 | ZntR promoter without RBS in front of the GVP cluster | None | ~6150 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.38 | Restriction(+) + Sequenced (3 mutations) |
| ArsR- + GVP in J61035 | ArsR promoter without RBS in front of the GVP cluster | None | ~6150 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.39 | Restriction(+) + Sequenced Okay |
| ArsR- in J61002 | pArsR (promoter without RBS) | 71 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.40 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ZntR- in J61002 | pZntR (promoter without RBS) | 65 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.41 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations) | |
| CueO- in J61002 | pCueO (promoter without RBS) | 43 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.42 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ZntR+RBS in J61002 | pZntR (promoter with own RBS) | 91 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.43 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ArsR+RBS in J61002 | pArsR (promoter with own RBS) | 81 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.44 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| CueO+RBS in J61002 | pCueO (promoter with own RBS) | 71 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.45 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| SmtA-GST in pGEX-3x | SmtA-GST fusion | no biobrick primers available | 1400 | [http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x] | Ampicillin | plate 3, pos. 46 | PCR, restriction |
| SmtA in pET29a | SmtA | 900 | [http://www.merckbiosciences.co.uk/product/69871 pET29a] | Kanamycin | plate 3, pos. 47 | PCR, restriction | |
| MymT in pGB68 | MymT | 215 | [http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB) | Ampicillin | plate 3, pos. 48 | PCR, restriction | |
| GlpF and fMT in pMAL-MT | GlpF and fMT on a synthetic operon in the vector | and | fMT = 200, GlpF = 900? | [http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB) | Ampicillin | plate 3, pos. 49 | PCR, restriction |
| MBP-ArsR | MBP-ArsR Fusion protein | 15180 | Ampicillin/chloramphenicol | plate 3, pos. 53 | Restriction analysis] & PCR | ||
| pNL29 | GVP Gene cluster | 6000 | [http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS] | Ampicillin | plate 3, pos. 54 | Buoyancy phenotype | |
| pLac-fMT | Metallothionein with inducible promotor | 430 | Ampicillin/chloramphenicol | plate 3, pos. 55 | Quality control | ||
| pLow-fMT #4 | Metallothionein with low constitutive promotor | 265 | Ampicillin/chloramphenicol | plate 3, pos. 56 | Quality control | ||
| pLow-fMT #3 | Metallothionein with low constitutive promotor | 265 | Ampicillin/chloramphenicol | plate 3, pos. 57 | Quality control | ||
| SmtA | Metallothionein | 678 | Ampicillin/chloramphenicol | plate 3, pos. 58 | Quality control | ||
| fMT #6 | Metallothionein for As3+ | 222 | Ampicillin/chloramphenicol | plate 3, pos. 59 | Sequence 2 deletions between EcoRI and XbaI sites | ||
| fMT #4 | Metallothionein for As3+ | 222 | Ampicillin/chloramphenicol | plate 3, pos. 60 | Sequence Okay! | ||
| High consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 75 | Quality control | ||
| Medium consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 76 | Quality control | ||
| Low consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 77 | Quality control | ||
| High consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 78 | Quality control | ||
| Medium consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 79 | Quality control | ||
| Low consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 80 | Quality control | ||
| Reference constitutive promoter) | Constitutive promoter | ? | Base Pairs (bp) | ? | ? | plate 3, pos. 81 | Quality control |
