Team:EPF-Lausanne/Protocols/1.5 steps PCR

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'''1.''' Do the following mixing:
'''1.''' Do the following mixing:
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{| class="wikitable" style="text-align:center; width:30%;"
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{| class="wikitable" style="text-align:left; width:30%;"
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! scope=row | dNTP
! scope=row | dNTP
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'''2.''' Make it run '''10''' cycles:
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{| class="wikitable" style="text-align:left; width:30%;"
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|-
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! scope=row | 1
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|94°C
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|4:00
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|-
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! scope=row | 2
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|94°C
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|0:30
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|-
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! scope=row | 3
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|55°C
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|1:00
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|-
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! scope=row | 4
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|72°C
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|2:00
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|-
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! scope=row | 5
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|Cycle 2-4 10 times
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|
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|-
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! scope=row | 6
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|72°C
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|7:00
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|-
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! scope=row | 7
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|4°C
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|&infin;
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|}
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Revision as of 09:41, 4 September 2009



Miniprep Protocol




1. Do the following mixing:

dNTP 1 μl
5x Buffer + MgCl2 10 μl
Primer Mix 1 μl
template 0.5 μl
HiFi Plus DNAp 0.5 μl
dH2O 36.5 μl
Final Volume 50 μl


2. Make it run 10 cycles:

1 94°C 4:00
2 94°C 0:30
3 55°C 1:00
4 72°C 2:00
5 Cycle 2-4 10 times
6 72°C 7:00
7 4°C ∞


Retrieved from "http://2009.igem.org/Team:EPF-Lausanne/Protocols/1.5_steps_PCR"