Virginia Commonwealth/4 June 2009
From 2009.igem.org
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! Control !! Plate Type !! Observation !! | ! Control !! Plate Type !! Observation !! | ||
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- | ! | + | ! NEB10β (+) |
| LB || lawn || | | LB || lawn || | ||
|- | |- | ||
- | ! | + | ! NEB10β (-) |
| Cm || no growth || | | Cm || no growth || | ||
|- | |- | ||
- | ! | + | ! NEB10β (-) |
| Amp || no growth || | | Amp || no growth || | ||
|- | |- | ||
- | ! | + | ! NEB10β (-) |
| Kan || no growth || | | Kan || no growth || | ||
|- | |- |
Revision as of 16:16, 7 June 2009
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Thursday 04 June 2009
Results
- Overnight 5mL culture of I13521 looks great (i.e., it's pinkish red) - GMcArthurIV 15:03, 4 June 2009 (UTC)
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
J13002 | Amp | 10s of colonies | n/a | |
PSB 1C3 | Cm | 10s of colonies | The colonies are pinkish in color because the rfp is being expressed. | |
R0040 | Amp | 10s of colonies | n/a | |
E1010 | Kan | 100s of colonies | n/a | |
PSB 4C5 | Cm | about 9 colonies | n/a | |
E0840 | Amp | 10s of colonies | n/a |
- Observations on the Controls
Control | Plate Type | Observation | |
---|---|---|---|
NEB10β (+) | LB | lawn | |
NEB10β (-) | Cm | no growth | |
NEB10β (-) | Amp | no growth | |
NEB10β (-) | Kan | no growth | |
DB3.1 (-) | Cm | no growth | |
DB3.1(+) | LB | lawn |
MandM 16:14, 7 June 2009 (UTC)
Tasks
- Lab orientation and safety overview
- Synthetic biology overview discussion
Wetlab
- Transform NEB10β or DB3.1 with the following parts and plate overnight:
Part No. | Type | ccdB gene | Resistance | Plate | Well | |
---|---|---|---|---|---|---|
[http://partsregistry.org/Part:BBa_R0040 R0040] | Promoter | No | AmpR | N/A | N/A | |
[http://partsregistry.org/Part:BBa_E0840 E0840] | GFP RET | No | AmpR | N/A | N/A | |
[http://partsregistry.org/Part:BBa_J13002 J13002] | Expression plasmid | No | AmpR | 1 | 13B | |
[http://partsregistry.org/Part:BBa_E1010 E1010] | RFP CDS | No | KanR | 1 | 18F | |
[http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3] | High-copy assembly plasmid | No | CmR | 1 | 5A | |
[http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5] | Low-copy assembly plasmid | Yes | CmR | 1 | 5I |
- Controls (electroporate NEB10β or DB3.1 without plasmid DNA and grow on agar overnight):
Part No. | Type | Resistant to ccdB | Resistance | |
---|---|---|---|---|
(+) Control | NEB10β on LB | No | None | |
(-) Control | NEB10β on LB+Amp | No | None | |
(-) Control | NEB10β on LB+Cm | No | None | |
(-) Control | NEB10β on LB+Kan | No | None | |
(-) Control | DB3.1 on LB+Cm | Yes | None | |
(+) Control | DB3.1 on LB+Kan | Yes | None |