Team:Newcastle/Labwork/9 September 2009

From 2009.igem.org

(Difference between revisions)
(→Introduction)
(→Metal Sensing Team)
Line 10: Line 10:
'''Today's task is to tranform ''DH5-alpha E.coli'' bacteria with our newly synthesized BioBricks - one set of cells should be tranformed with the ''cotC-GFP-smtA'' BioBrick and the other group of cells should be transformed with the ''kinA'' BioBrick'''
'''Today's task is to tranform ''DH5-alpha E.coli'' bacteria with our newly synthesized BioBricks - one set of cells should be tranformed with the ''cotC-GFP-smtA'' BioBrick and the other group of cells should be transformed with the ''kinA'' BioBrick'''
-
 
+
===Procedure===
 +
To carry out the transformations of the two sets of ''DH5-alpha E.coli'' cells with our two BioBricks, we adhered to [https://2009.igem.org/Team:Newcastle/Project/Labwork/PhilsProtocols#Transforming_DNA_.22Phil_Style.22 Dr. Aldridge's transformation by heat shock protocol].
{{:Team:Newcastle/Footer}}
{{:Team:Newcastle/Footer}}
{{:Team:Newcastle/Right}}
{{:Team:Newcastle/Right}}

Revision as of 22:22, 13 September 2009

Lab Work - 09/09/09

Metal Sensing Team

Introduction

In the last lab session the Metal Sensing Team had shown that the version of the BBa_J33206 BioBrick submitted by Chris French (contained within the pSB1A2 plasmid) was indeed the correct one. However any further work on this BioBrick should now be put on temporary hold because of the arrival of our newly synthesized BioBricks. These synthesized BioBricks need to be transformed in E. coli bacteria as well as other processing measures.

Today's task is to tranform DH5-alpha E.coli bacteria with our newly synthesized BioBricks - one set of cells should be tranformed with the cotC-GFP-smtA BioBrick and the other group of cells should be transformed with the kinA BioBrick

Procedure

To carry out the transformations of the two sets of DH5-alpha E.coli cells with our two BioBricks, we adhered to Dr. Aldridge's transformation by heat shock protocol.



News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
Retrieved from "http://2009.igem.org/Team:Newcastle/Labwork/9_September_2009"