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Team:Newcastle/Labwork/10 September 2009
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==Metal Sensing Team== | ==Metal Sensing Team== | ||
===Introduction=== | ===Introduction=== | ||
| + | Yesterday saw the Metal Sensing team attempt to transform ''DH5-alpha E.coli'' bacterial cells with two BioBricks (the ''cotC-GFP-smtA'' BioBrick and the ''kinA'' BioBrick) and plate them out on LB + kanamycin plates. The ''cotC-GFP-smtA'' BioBrick is part of the Metal Sensing team's sub-project and the ''kinA'' BioBrick will be used by the Stochastic Switch team's sub-project. | ||
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| + | '''Today's exercise should see the Metal Sensing team inoculate LB medium with any transformant cultures which grow on these plates - if there are no cultures present then the transformations will be attempted a second time.''' | ||
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| + | ===Observations=== | ||
| + | On all four plates there could be seen hundreds of transformant colonies - this was to be expected as the DNA used to transform the ''DH5-alpha'' cells was in pure form. | ||
| + | |||
| + | ===Procedure=== | ||
{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} | ||
Revision as of 23:01, 13 September 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Lab Work - 10/09/09
Metal Sensing Team
Introduction
Yesterday saw the Metal Sensing team attempt to transform DH5-alpha E.coli bacterial cells with two BioBricks (the cotC-GFP-smtA BioBrick and the kinA BioBrick) and plate them out on LB + kanamycin plates. The cotC-GFP-smtA BioBrick is part of the Metal Sensing team's sub-project and the kinA BioBrick will be used by the Stochastic Switch team's sub-project.
Today's exercise should see the Metal Sensing team inoculate LB medium with any transformant cultures which grow on these plates - if there are no cultures present then the transformations will be attempted a second time.
Observations
On all four plates there could be seen hundreds of transformant colonies - this was to be expected as the DNA used to transform the DH5-alpha cells was in pure form.
Procedure
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
