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Team:Groningen/Notebook/15 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== '''Planning''' :→ {{todo}} work out the wiki page for GVP :→ {{todo}} make a doodle for presentation planning (1-19...) |
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| Line 11: | Line 11: | ||
:→ {{todo}} media attention | :→ {{todo}} media attention | ||
| - | :→ {{todo}} | + | :→ {{todo}} |
| + | |||
| + | '''Plates''' | ||
| + | |||
| + | The | ||
| + | |||
| + | {|cellpadding="2" cellspacing="1" border="4" | ||
| + | |'''Name''' | ||
| + | |'''Plasmid Used''' | ||
| + | |'''Antibiotics on Plasmid''' | ||
| + | |'''No. of Colonies''' | ||
| + | |'''Date''' | ||
| + | |- | ||
| + | |GlpF no.1 (low) | ||
| + | |pSB1AC3 | ||
| + | |Ampicillin | ||
| + | |0 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |GlpF no.1 (high) | ||
| + | |pSB1AC3 | ||
| + | |Ampicillin | ||
| + | |2 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |GlpF no.2 (low) | ||
| + | |pSB1AC3 | ||
| + | |Ampicillin | ||
| + | |1 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |GlpF no.2 (high) | ||
| + | |pSB1AC3 | ||
| + | |Ampicillin | ||
| + | |1 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |pLacI (low) | ||
| + | |pSB1AC3 | ||
| + | |Ampicillin | ||
| + | |~30 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |pLacI (high) | ||
| + | |pSB1AC3 | ||
| + | |Ampicillin | ||
| + | |~80 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |pLacI-GVP no.1 (low) | ||
| + | |pSB1A2 | ||
| + | |Ampicillin | ||
| + | |0 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |pLacI-GVP no.1 (high) | ||
| + | |pSB1A2 | ||
| + | |Ampicillin | ||
| + | |4 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |pLacI-GVP no.2 (low) | ||
| + | |pSB1A2 | ||
| + | |Ampicillin | ||
| + | |0 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |pLacI-GVP no.2 (high) | ||
| + | |pSB1A2 | ||
| + | |Ampicillin | ||
| + | |9 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |Positive (J23101) (high) | ||
| + | |J61002 | ||
| + | |Ampicillin | ||
| + | |~1500 | ||
| + | |14 sept. | ||
| + | |- | ||
| + | |Negative (MQ) (high) | ||
| + | |MQ | ||
| + | |Ampicillin | ||
| + | |0 | ||
| + | |14 sept. | ||
| + | |} | ||
| + | |||
| + | |||
| + | :→ The plates showed a low amount of colonies, and also red in the case of the positive plate. | ||
| + | |||
| + | |||
| + | :→ The two plates with stripes of pZntR-GVP (pSB2K3) and pCueO-GVP (pSB2K3) culture for glycerol stocks showed growth and some single colonies. | ||
| + | |||
| + | |||
| + | :→ All plates were stored in the fridge for further use. | ||
| + | |||
| + | |||
| + | '''Cultures''' | ||
| + | |||
| + | The overnight cultures with LB-amp<sub>100</sub> medium of colonies E.coli TOP10 with | ||
===Transporters=== | ===Transporters=== | ||
Revision as of 08:38, 15 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
GVP Cluster
Planning
- → TODO work out the wiki page for GVP
- → TODO make a doodle for presentation planning (1-19 oct.)
- → TODO media attention
- → TODO
Plates
The
| Name | Plasmid Used | Antibiotics on Plasmid | No. of Colonies | Date |
| GlpF no.1 (low) | pSB1AC3 | Ampicillin | 0 | 14 sept. |
| GlpF no.1 (high) | pSB1AC3 | Ampicillin | 2 | 14 sept. |
| GlpF no.2 (low) | pSB1AC3 | Ampicillin | 1 | 14 sept. |
| GlpF no.2 (high) | pSB1AC3 | Ampicillin | 1 | 14 sept. |
| pLacI (low) | pSB1AC3 | Ampicillin | ~30 | 14 sept. |
| pLacI (high) | pSB1AC3 | Ampicillin | ~80 | 14 sept. |
| pLacI-GVP no.1 (low) | pSB1A2 | Ampicillin | 0 | 14 sept. |
| pLacI-GVP no.1 (high) | pSB1A2 | Ampicillin | 4 | 14 sept. |
| pLacI-GVP no.2 (low) | pSB1A2 | Ampicillin | 0 | 14 sept. |
| pLacI-GVP no.2 (high) | pSB1A2 | Ampicillin | 9 | 14 sept. |
| Positive (J23101) (high) | J61002 | Ampicillin | ~1500 | 14 sept. |
| Negative (MQ) (high) | MQ | Ampicillin | 0 | 14 sept. |
- → The plates showed a low amount of colonies, and also red in the case of the positive plate.
- → The two plates with stripes of pZntR-GVP (pSB2K3) and pCueO-GVP (pSB2K3) culture for glycerol stocks showed growth and some single colonies.
- → All plates were stored in the fridge for further use.
Cultures
The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with
Transporters
Metal Accumulation
Vectors
Dry
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