Team:BIOTEC Dresden/Project v2

From 2009.igem.org

(Difference between revisions)
Line 1: Line 1:
{{:Team:BIOTEC_Dresden/NewTemplate}}
{{:Team:BIOTEC_Dresden/NewTemplate}}
-
=== Day 0 ===
+
=== Temporal and spatial control of protein synthesis by in vitro
 +
recombination inside picoliter reactors  ===
-
Here we have a new wiki page.... Let's check if we can modify the template.
+
Manufacturing functionalized proteins in vitro poses a challenge, as
-
 
+
it requires coordinated molecular assemblies and multi-step reactions.  
-
Yay, works. Now, it's time to clean this up.
+
In this project we aim to control, over time and space, the production
-
 
+
of proteins tagged with a silver-binding peptide for in situ silver
-
 
+
nanoparticle nucleation inside microdroplets generated by microfluidic
-
=== Day 0.5 ===
+
devices. Combining a transcription-translation system with protein
-
 
+
coding genes and a recombination logic inside microdroplets provides
-
Let's see... we have some additional floating boxes for content like links and news.
+
spatial control. Moreover, in the microfluidic chamber we can pinpoint
-
 
+
the beginning of synthesis, and easily track and isolate the droplets.  
-
The question now is: what exactly do we want to put there?
+
Site-specific recombination generates a molecular timer for temporal
-
 
+
control of protein synthesis. Unlike transcriptional regulation, this  
-
And why is this white box so limited?
+
method gives true all-or-none induction due to covalent modification
-
 
+
of DNA by Flp recombinase. Determining the transfer curve of inter-FRT
-
=== Day 0.6 ===
+
site distance versus average recombination time allows the onset of  
-
+
gene expression to be predicted. We then apply this Flp reporter
-
Quite a lot of problems arise from a mistakenly places foot(er)...
+
system as a powerful PoPS measurement device.  
-
 
+
-
=== Day 1 ===
+
-
 
+
-
Set up a color scheme, it's still not really convincing. The opacity of the biotec logo has to be corrected, too. Oh, and the header code has to be cleaned up.
+
{{:Team:BIOTEC_Dresden/NewTemplateEnd}}
{{:Team:BIOTEC_Dresden/NewTemplateEnd}}

Revision as of 19:15, 19 September 2009

=== Temporal and spatial control of protein synthesis by in vitro recombination inside picoliter reactors ===

Manufacturing functionalized proteins in vitro poses a challenge, as it requires coordinated molecular assemblies and multi-step reactions. In this project we aim to control, over time and space, the production of proteins tagged with a silver-binding peptide for in situ silver nanoparticle nucleation inside microdroplets generated by microfluidic devices. Combining a transcription-translation system with protein coding genes and a recombination logic inside microdroplets provides spatial control. Moreover, in the microfluidic chamber we can pinpoint the beginning of synthesis, and easily track and isolate the droplets. Site-specific recombination generates a molecular timer for temporal control of protein synthesis. Unlike transcriptional regulation, this method gives true all-or-none induction due to covalent modification of DNA by Flp recombinase. Determining the transfer curve of inter-FRT site distance versus average recombination time allows the onset of gene expression to be predicted. We then apply this Flp reporter system as a powerful PoPS measurement device.


Retrieved from "http://2009.igem.org/Team:BIOTEC_Dresden/Project_v2"