Virginia Commonwealth/11 June 2009
From 2009.igem.org
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- | * | + | * Literature Discussion |
- | * | + | * Discussion of iGEM ideas |
====Wetlab==== | ====Wetlab==== | ||
- | * | + | * Picking Colonies to be grown for Mini-preps and two Maxi-preps. |
- | * | + | * |
Revision as of 22:55, 11 June 2009
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Contents |
Thursday 11 June 2009
Results
- Note these were plated Wednesday evening with two groups splitting the work.
- Group 1: Craig, Clay, Afton plates
- Observations on the Controls
Control | Plate Type | Observation | ||
---|---|---|---|---|
NEB10β (+) | LB | lawn | ||
NEB10β (-) | Cm | no growth | ||
NEB10β (-) | Amp | no growth | ||
NEB10β (-) | Kan | 3 colonies | The possibilities for this are a non-homogeneous medium with very small sections capable of sustaining life or there was degradation of the Kan-selective medium. |
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
pSB 3K3 | Kan | 10s of colonies | n/a | |
E0240 | Amp | 10s of colonies | ||
J23113 | Amp | 10s of colonies | n/a | |
J23116 | Amp | 100s of colonies | n/a | |
J23101 | Amp | about 9 colonies | n/a | |
R0040 | Amp | 10s of colonies | n/a |
- Group 2: Adam, Kevin and Maria Plates
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
R0011 | Amp | about 9 colonies | n/a | |
J23100 | Amp | 10s of colonies | n/a | |
J23103 | Amp | 10s of colonies | The colonies are pinkish in color because the rfp is being expressed. | |
J23104 | Amp | 10s of colonies | n/a | |
J23105 | Amp | 100s of colonies | n/a | |
J23106 | Amp | about 9 colonies | n/a | |
J23107 | Amp | 10s of colonies | n/a |
- 2
MandM 22:52, 11 June 2009 (UTC)
Tasks
- Literature Discussion
- Discussion of iGEM ideas
Wetlab
- Picking Colonies to be grown for Mini-preps and two Maxi-preps.