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Team:Warsaw/Primers
From 2009.igem.org
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|style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro-box cro-box] | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro-box cro-box] | ||
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| - | |<html><a name="invF">invF</a></html> | + | |<html><a name="invF">invF (- overhang)</a></html> |
|ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | |ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | ||
|style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
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| - | |<html><a name="invR">invR</a></html> | + | |<html><a name="invR">invR (- overhang)</a></html> |
|GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | |GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | ||
| + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
| + | |- | ||
| + | |<html><a name="invF">invF (+ overhang)</a></html> | ||
| + | |GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | ||
| + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
| + | |- | ||
| + | |<html><a name="invR">invR (+ overhang)</a></html> | ||
| + | |CTGCAGCGGCCGCTACTAGTACTATTGAGGCTCCGCACACAG | ||
|style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
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Revision as of 14:57, 29 September 2009
Primers
Here is the list of primers we have been using during our Project.
cloning primers
| name | sequence | description |
|---|---|---|
| croboxF | ATCTAGATACCTCTGGCGGTGATACTAGTGT | forward primer for cro-box |
| croboxR | ACACTAGTATCACCGCCAGAGGTATCTAGAT | reverse primer for cro-box |
| invF (- overhang) | ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | forward primer for invasin |
| invR (- overhang) | GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | reverse primer for invasin |
| invF (+ overhang) | GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | forward primer for invasin |
| invR (+ overhang) | CTGCAGCGGCCGCTACTAGTACTATTGAGGCTCCGCACACAG | reverse primer for invasin |
| hlyF | TGTCTAGAATGAAAAAAATAATGCTAGTT | forward primer for listeriolysin |
| hlyR | GACTAGTTTATTCGATTGGATTATCTA | reverse primer for listeriolysin |
| croF | ATCTAGAATGGAACAACGCATAACC | forward primer for cro |
| croR | GTACTAGTGGGCTGGAATGTGTAAG | reverse primer for cro |
| phoF | ATCTAGAATGATGCGCGTACTGGTTGTAG | forward primer for phoP/phoQ |
| phoR | TTACTAGTGATGCGTGGAAGAACGCACAG | reverse primer for PhoP/PhoQ |
| mgtCF | ATCTAGATCCATTTTTTCTGGAAGAATAAG | forward primer for the mgtc gene promoter |
| mgtCR | GTACTAGTCCTCGTCATGTTGTTTTATTTTTT | reverse primer for the mgtc gene promoter |
| p53F | GCTCTAGAATGGAGGAGCCGCAGTC | forward primer for p53 |
| p53R | GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | reverse primer for p53 |
| phoPF | gtttcttcgaattcgcggccgcttctagatgatgcgcgtactggttgtagag | forward primer for PhoP (new) |
| phoPR | gtttcttcctgcagcggccgctactagtattagcgcaattcaaaaagatatccttgtcc | reverse primer for PhoP (new) |
| phoQF | gtttcttcgaattcgcggccgcttctagagatgaataaatttgctcgccattttctgc | forward primer for PhoQ (new) |
| phoQR | gtttcttcctgcagcggccgctactagtattattcctctttctgtgtgggatgctgtc | reverse primer for PhoQ (new) |
| baxF | ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | forward primer for bax |
| baxR | ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | reverse primer for bax |
| invF | GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | forward primer for invasin (with completely prefix and sufix) |
| invR | TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | reverse primer for invasin (with completely prefix and sufix) |
| inlapsF | GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG | forward primer for internalin A |
| inlapsR | GCGCTGCAGTTATTTACTAGCACGTGCTT | reverse primer for internalin A |
| JPSwiF | TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC | |
| JPSwiRbez | GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC | |
| JPSwiRGFP | GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG | |
| mgtCnewF | GAATTCGCGGCCGCTTCTAGAGTCCATTTTTTCTGGAAGAATAAG | revised forward primer for the mgtc gene promoter (now with complete prefix) |
| mgtCnewR | CTGCAGCGGCCGCTACTAGTACCTCGTCATGTTGTTTTATTTTTTT | revised reverse primer for the mgtc gene promoter (now with complete suffix) |
| YFPsilverF | GATGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAG | forward primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
| YFPsilverR | GCTCTGCAGCGGCCGCTACTAGTAGCTACTAAAGCGTAGTT | reverse primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
| mitoCOXsilverF | TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | forward primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
| mitoCOXsilverR | TTCCTGCAGCGGCCGCTACTAGTCAACGAATGGATCTTGGCGCGC | reverse primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
| p53silverF | CGAGAATTCGCGGCCGCTTCTAGAATGGAGGAGCCGCAGTC | forward primer for p53 CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
| p53silverR | TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | reverse primer for p53 CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
| secr_silverF | GGGAATTCCGCGGCCGCTTCTAGAGATGGCGGAGAAGGGAATG | forward primer for hemolysin secretion signal compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
| secr_silverR | CGGCTGCAGCGGCCGCTACTAGTTTATGCTGATGCTGTCAAAGTTATTG | reverse primer for hemolysin secretion signal compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] |
