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Team:Groningen/Project
From 2009.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
Planning and requirements: | Planning and requirements: | ||
* Gasvesicle: | * Gasvesicle: | ||
| - | ** | + | **Modelling: |
| - | *** | + | *** Bouyancy |
**** Permeability | **** Permeability | ||
| - | **** | + | **** number of gasvesicles |
| - | **** | + | **** Where do the gasvesciles end, in hight. |
| - | *** | + | *** Mass of the bacteria (e.coli) |
| - | *** | + | *** How long it takes before it floates |
| - | **** | + | **** how long it take untill it is being expressed |
| - | **** | + | **** How long it will take untill there are enough gasvesicles |
| - | **** | + | **** how does it stay floating |
** Lab | ** Lab | ||
*** GvP | *** GvP | ||
| - | **** Cluster | + | **** Cluster of biobricks |
| - | **** Vector | + | **** Vector ordered from article |
| - | **** | + | **** first expression with constutatieve promotor, later with metal sensitive promotor |
| - | *** | + | *** Measurements |
| - | **** | + | **** Where are the bacteria, what are bac concentrations on a certain hight |
| - | ***** | + | ***** Making pictures on certain hight and compare with picture of known concentration |
* Metal importers | * Metal importers | ||
| - | ** | + | ** Modelling |
| - | *** Import | + | *** Import speed |
| - | *** | + | *** Amount |
*** Max | *** Max | ||
** Lab | ** Lab | ||
*** HmtA | *** HmtA | ||
| - | **** Zn/Cu | + | **** Zn/Cu alone |
| - | **** B-type ATPase ( | + | **** B-type ATPase (could be use if there is a ATP shortage?) |
*** CitM | *** CitM | ||
| - | **** | + | **** Divalent ions |
| - | **** | + | **** Citrate around |
| - | **** | + | **** Citrate can bind metals that are already bound. |
| - | *** | + | *** Measurements |
| - | **** Transporter, | + | **** Transporter, on/off mechanism, up to what concentration (in the cell) does it still have metal uptake. |
| - | **** | + | **** Measure concentration of metal. difference between begin and end concentrations of metal outside the cell |
* Promotors | * Promotors | ||
| - | ** | + | ** Modelling |
| - | *** | + | *** How do they react to metal, metal concentration vs expression of the protein after promotor (bv GFP) |
** Lab | ** Lab | ||
| - | *** | + | *** Measuments |
| - | **** | + | **** Expression of the gene behind the promotor(GFP) |
*** Cu-promotor | *** Cu-promotor | ||
*** Arc promotor | *** Arc promotor | ||
| - | * | + | * Accumulation |
| - | ** | + | ** Modelling |
| - | *** | + | *** Speed |
| - | *** | + | *** Metaliotheines concentration |
** Lab | ** Lab | ||
| - | *** | + | *** Measurements |
| - | ***** | + | ***** Measure accumulation. By measuring before/after concentration metal with and without accumulation protein. |
*** Biobrick Bba_K129004 | *** Biobrick Bba_K129004 | ||
*** Rest | *** Rest | ||
| Line 68: | Line 68: | ||
Order of action | Order of action | ||
| - | # | + | # Bouyancy |
| - | # | + | # Metal importation |
| - | # | + | # Accumulation |
# Metal sensitive promotor | # Metal sensitive promotor | ||
Revision as of 15:56, 14 June 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| The Project
|
|---|
Planning and requirements:
- Gasvesicle:
- Modelling:
- Bouyancy
- Permeability
- number of gasvesicles
- Where do the gasvesciles end, in hight.
- Mass of the bacteria (e.coli)
- How long it takes before it floates
- how long it take untill it is being expressed
- How long it will take untill there are enough gasvesicles
- how does it stay floating
- Bouyancy
- Lab
- GvP
- Cluster of biobricks
- Vector ordered from article
- first expression with constutatieve promotor, later with metal sensitive promotor
- Measurements
- Where are the bacteria, what are bac concentrations on a certain hight
- Making pictures on certain hight and compare with picture of known concentration
- Where are the bacteria, what are bac concentrations on a certain hight
- GvP
- Modelling:
- Metal importers
- Modelling
- Import speed
- Amount
- Max
- Lab
- HmtA
- Zn/Cu alone
- B-type ATPase (could be use if there is a ATP shortage?)
- CitM
- Divalent ions
- Citrate around
- Citrate can bind metals that are already bound.
- Measurements
- Transporter, on/off mechanism, up to what concentration (in the cell) does it still have metal uptake.
- Measure concentration of metal. difference between begin and end concentrations of metal outside the cell
- HmtA
- Modelling
- Promotors
- Modelling
- How do they react to metal, metal concentration vs expression of the protein after promotor (bv GFP)
- Lab
- Measuments
- Expression of the gene behind the promotor(GFP)
- Cu-promotor
- Arc promotor
- Measuments
- Modelling
- Accumulation
- Modelling
- Speed
- Metaliotheines concentration
- Lab
- Measurements
- Measure accumulation. By measuring before/after concentration metal with and without accumulation protein.
- Biobrick Bba_K129004
- Rest
- Measurements
- Modelling
Order of action
- Bouyancy
- Metal importation
- Accumulation
- Metal sensitive promotor
