Virginia Commonwealth/12 June 2009
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[[User:MandM|MandM]] 19:01, 12 June 2009 (UTC) | [[User:MandM|MandM]] 19:01, 12 June 2009 (UTC) | ||
**Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing. | **Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing. | ||
+ | -[[User:Bussingkm|Bussingkm]] 15:40, 13 June 2009 (UTC) |
Revision as of 15:40, 13 June 2009
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Contents |
Friday 12 June 2009
Results
- Data from plasmid purification
- Buffer EB was used instead of water and 500 microliters was used instead of 5 microliters so the DNA will have to be concentrated.
- 114 microliters of buffer and 6 microliters of sample
Part No. | A260 (nm) | A280 (nm) | A260/A280 | Concentration (microgram/microliter) | Amount (microgram/milliliter) | Volume (microliter) | |
---|---|---|---|---|---|---|---|
J23103 | .007 | .003 | 2.33 | 7.0 | 2.52 | 360 | |
J23105 | .008 | .004 | 2.00 | 8.0 | 2.96 | 370 | |
J23100 | .014 | .011 | 1.273 | 14.0 | 5.32 | 380 | |
J23104 | .011 | .009 | 1.22 | 11.0 | 4.18 | 380 | |
J23101 | .012 | .008 | 1.5 | 12.0 | 4.56 | 380 | |
J23107 | .018 | .014 | 1.286 | 18.0 | 8.1 | 450 | |
J23116 | .020 | .016 | 1.25 | 20.0 | 7.2 | 360 | |
J23113 | .014 | .010 | 1.4 | 14.0 | 5.18 | 370 | |
J23110 | .012 | .007 | 1.714 | 12.0 | 4.2 | 350 |
- 100 microliters of buffer and 20 microliters of sample
Part No. | A260 (nm) | A280 (nm) | A260/A280 | Concentration (microgram/microliter) | Amount (microgram) | Volume (microliter) | |
---|---|---|---|---|---|---|---|
J23102 | .031 | .025 | 1.24 | 31.0 | 10.23 | 330 | |
R0011 | .021 | .016 | 1.313 | 21.0 | 5.46 | 260 | |
R0040 | .015 | .011 | 1.364 | 15.0 | 5.4 | 360 |
MandM 19:17, 12 June 2009 (UTC)
Tasks
- Review Literature
Wetlab
- Mini-Prep on the promoter.
- Maxi-Prep on the reporter and the backbone.
MandM 19:01, 12 June 2009 (UTC)
- Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing.
-Bussingkm 15:40, 13 June 2009 (UTC)