Team:Newcastle/Labwork/24 July 2009

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=Lab session: 24th July=
=Lab session: 24th July=
==Introduction==
==Introduction==
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The previous lab session has seen the team make a start on the real lab work and also beginning to finish the work involved with the introductory lab sessions. To begin the real lab work five BioBricks from the Spring Distribution were hydrated and stored in the freezer, namely BioBricks ''BBA_C0056'' (cI in the lambda phage), ''BBa_B1002'' (terminator sequence), ''BBa_C0077'' (CinR activator), ''BBa_C0076'' (autoinducer synthetase) and ''BBa_R0077'' (promoter which is CinR and HSL regulated - RBS+)
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The previous lab session has seen the team make a start on the real lab work and also beginning to finish the work involved with the introductory lab sessions.  
 +
 
 +
To begin the real lab work five BioBricks from the Spring Distribution were hydrated and stored in the freezer, namely BioBricks ''BBA_C0056'' (cI in the lambda phage), ''BBa_B1002'' (terminator sequence), ''BBa_C0077'' (CinR activator), ''BBa_C0076'' (autoinducer synthetase) and ''BBa_R0077'' (promoter which is CinR and HSL regulated - RBS+)
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The real lab work also involved the team inoculating 2 flasks of 100ml LB with ''E. coli'' containing ''pMUTIN4'' and growing the cultures overnight in the 37ºC orbital incubator.
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The introductory lab session work saw the team conduct the first few stages of the ethanol precipitation of the ''GFP'' and ''RFP'' midi-prep samples.
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Revision as of 10:43, 28 September 2009

Lab session: 24th July

Introduction

The previous lab session has seen the team make a start on the real lab work and also beginning to finish the work involved with the introductory lab sessions.

To begin the real lab work five BioBricks from the Spring Distribution were hydrated and stored in the freezer, namely BioBricks BBA_C0056 (cI in the lambda phage), BBa_B1002 (terminator sequence), BBa_C0077 (CinR activator), BBa_C0076 (autoinducer synthetase) and BBa_R0077 (promoter which is CinR and HSL regulated - RBS+)

The real lab work also involved the team inoculating 2 flasks of 100ml LB with E. coli containing pMUTIN4 and growing the cultures overnight in the 37ºC orbital incubator.

The introductory lab session work saw the team conduct the first few stages of the ethanol precipitation of the GFP and RFP midi-prep samples.



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