Revision as of 15:04, 29 September 2009

Meetings Calendar

Lab Work - 29/07/09

Introduction

In yesterday's lab session we conducted mini-preps for three sets of JM109 E. coli cells which contained BioBricks BBa_C0056, BBa_B1002 and BBa_R0077 (seen as we picked three colonies for each of the BioBrick trasnformants there was a total of nine mini-preps conducted). A sample of these prepped plasmids were then run on agarose gel; although the undigested plasmid samples couldn't determine whether the DNA was in fact the BioBrick incorporated within the correct plasmid the gel certainly showed that the samples were consistent (trulin out contamination).

The next step in the lab plan is to digest samples of these 9 mini-preps and then run these fragments on agarose gel by DNA Gel Electrophoresis. As well as this, the team needs to consider another attempt at transforming E. coli with the BioBricks BBa_C0077 and BBa_C0076 (the transformations which didn't yield colonies on LB + ampicillin plates). In addition the team needs to start to freeze down cells which will probably be needed in the future.

Practical Outline

Procedure

Results

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

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@@ Line 5: / Line 5: @@
 =Lab Work - 29/07/09=
 ==Introduction==
+In yesterday's lab session we conducted mini-preps for three sets of ''JM109'' ''E. coli'' cells which contained BioBricks ''BBa_C0056'', ''BBa_B1002'' and ''BBa_R0077'' (seen as we picked three colonies for each of the BioBrick trasnformants there was a total of nine mini-preps conducted). A sample of these prepped plasmids were then run on agarose gel; although the undigested plasmid samples couldn't determine whether the DNA was in fact the BioBrick incorporated within the correct plasmid the gel certainly showed that the samples were consistent (trulin out contamination).
+The next step in the lab plan is to digest samples of these 9 mini-preps and then run these fragments on agarose gel by DNA Gel Electrophoresis. As well as this, the team needs to consider another attempt at transforming ''E. coli'' with the BioBricks ''BBa_C0077'' and BBa_C0076'' (the transformations which didn't yield colonies on LB + ampicillin plates). In addition the team needs to start to freeze down cells which will probably be needed in the future.
 ==Practical Outline==
 ==Procedure==

Team:Newcastle/Labwork/29 July 2009

From 2009.igem.org