Team:Newcastle/Labwork/30 July 2009

From 2009.igem.org

(Difference between revisions)
(→Introduction)
Line 1: Line 1:
 +
{{:Team:Newcastle/CSS}}
 +
{{:Team:Newcastle/Header}}
 +
{{:Team:Newcastle/Left}}
 +
__NOTOC__
=Lab Work - 30/07/09=
=Lab Work - 30/07/09=
==Introduction==
==Introduction==
Line 11: Line 15:
===Freezing Strains for TPA collection===
===Freezing Strains for TPA collection===
===Transforming ''E. coli'' with ''BBa_C0077'' and ''BBa_C0076''===
===Transforming ''E. coli'' with ''BBa_C0077'' and ''BBa_C0076''===
 +
{{:Team:Newcastle/Footer}}
 +
{{:Team:Newcastle/Right}}

Revision as of 18:28, 29 September 2009

Lab Work - 30/07/09

Introduction

In the previous lab session, a number of different things happened. The team digested the three sets of mini-preps originating from E. coli transformed with BBa_C0056, BBa_B1002 and BBa_R0077 with EcoRI and PstI; when run on agarose gel it could be seen that the correct DNA had been transformed!. Also in the previous lab session the team attempted a second transformation of JM109 E. coli cells with BioBricks BBa_C0077 and BBa_C0076. And finally the team chose some cells to be frozen down for long term storage and grew those selected cultures up in LB solutions.

Today's lab work will involve completing the freezing down bacteria procedure. It will also involve observing the LB + Kan plates for any BBa_C0077 and BBa_C0076 transformants; if there are colonies then mini-preps will be conducted and if the transformations have failed then a final attempt at transforming E. coli cells will commence. The team will also be given a lesson by PhD student Chris Birchall on how to pour LB agar plates the way in which Dr. Phil Aldridge pours plates.

Practical Outline

Observations

Procedure

Preparing and Pouring plates

Freezing Strains for TPA collection

Transforming E. coli with BBa_C0077 and BBa_C0076



News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
Retrieved from "http://2009.igem.org/Team:Newcastle/Labwork/30_July_2009"