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Team:Newcastle/Labwork/30 July 2009
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==Practical Outline== | ==Practical Outline== | ||
| + | These are the tasks the team need to complete by today: | ||
| + | <br> | ||
| + | # Observe ''BBa_C0077'' and ''BBa_C0076'' transformants on LB + kan plates | ||
| + | ## If successful inoculate 5ml LB with colonies for mini-preps tomorrow | ||
| + | ## If failed conduct a final set of ''E. coli'' transformations | ||
| + | # Prepare and pour some LB agar plates according to Dr. Phil Aldridge's method | ||
| + | # Freeze down cells for TPA collection | ||
| + | <br> | ||
| + | |||
==Observations== | ==Observations== | ||
==Procedure== | ==Procedure== | ||
Revision as of 19:04, 29 September 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Lab Work - 30/07/09
Introduction
In the previous lab session, a number of different things happened. The team digested the three sets of mini-preps originating from E. coli transformed with BBa_C0056, BBa_B1002 and BBa_R0077 with EcoRI and PstI; when run on agarose gel it could be seen that the correct DNA had been transformed!. Also in the previous lab session the team attempted a second transformation of JM109 E. coli cells with BioBricks BBa_C0077 and BBa_C0076. And finally the team chose some cells to be frozen down for long term storage and grew those selected cultures up in LB solutions.
Today's lab work will involve completing the freezing down bacteria procedure. It will also involve observing the LB + Kan plates for any BBa_C0077 and BBa_C0076 transformants; if there are colonies then mini-preps will be conducted and if the transformations have failed then a final attempt at transforming E. coli cells will commence. The team will also be given a lesson by PhD student Chris Birchall on how to pour LB agar plates the way in which Dr. Phil Aldridge pours plates.
Practical Outline
These are the tasks the team need to complete by today:
- Observe BBa_C0077 and BBa_C0076 transformants on LB + kan plates
- If successful inoculate 5ml LB with colonies for mini-preps tomorrow
- If failed conduct a final set of E. coli transformations
- Prepare and pour some LB agar plates according to Dr. Phil Aldridge's method
- Freeze down cells for TPA collection
Observations
Procedure
Preparing and Pouring plates
Freezing Strains for TPA collection
Transforming E. coli with BBa_C0077 and BBa_C0076
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
