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EPF-Lausanne/1 October 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | ===Culture=== | ||
| + | For next morning miniprep and subsequently shipping for sequencing. | ||
| + | Clones : 3x RO1 #1/2/3, 4x RO2 #4/5/8/10, 4x LT #2/5/6/12, 3x LR # 2/5/7, 4x BB #1/3/5/6. Total : 36 tues in 5ml LB + corresponding antibiotic. | ||
| + | ===Culture=== | ||
| + | For next morning characterization. | ||
| + | |||
| + | For clones : | ||
| + | |||
| + | -RO2 #10 + BB6, clone #8 (name = DT 2) | ||
| + | |||
| + | -RO2 #4 + BB1, clone #3 (name = DT 1). | ||
| + | |||
| + | All from 2nd re-done glycerol stock, and in M9/minimal + AA + thiamine. | ||
| + | |||
| + | At 37°C overnight in Sebastian's lab. | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 15:08, 4 October 2009
Wet Lab
Culture
For next morning miniprep and subsequently shipping for sequencing.
Clones : 3x RO1 #1/2/3, 4x RO2 #4/5/8/10, 4x LT #2/5/6/12, 3x LR # 2/5/7, 4x BB #1/3/5/6. Total : 36 tues in 5ml LB + corresponding antibiotic.
Culture
For next morning characterization.
For clones :
-RO2 #10 + BB6, clone #8 (name = DT 2)
-RO2 #4 + BB1, clone #3 (name = DT 1).
All from 2nd re-done glycerol stock, and in M9/minimal + AA + thiamine.
At 37°C overnight in Sebastian's lab.
People in the lab
Tu, Basile
