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Team:Groningen/Notebook/17 June 2009
From 2009.igem.org
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(New page: {{Team:Groningen/Notebook/Day| We transformed E. coli TOP10 with the following BioBricks (first diluted in 15ul diH2O, and 2ul was taken for transformation) *gvp (BBa_I750016, Plate 2, wel...) |
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{{Team:Groningen/Notebook/Day| | {{Team:Groningen/Notebook/Day| | ||
| - | We transformed E. coli TOP10 with the following BioBricks (first diluted in 15ul diH2O, and 2ul was taken for transformation) | + | *We transformed E. coli TOP10 with the following BioBricks (first diluted in 15ul diH2O, and 2ul was taken for transformation) |
| - | *gvp (BBa_I750016, Plate 2, well 11A) | + | **gvp (BBa_I750016, Plate 2, well 11A) |
| - | *RBS (BBa_B0034, Plate 1, well 2M) | + | **RBS (BBa_B0034, Plate 1, well 2M) |
| - | *Terminator (BBa_B0014, Plate 2, well 24C) | + | **Terminator (BBa_B0014, Plate 2, well 24C) |
| - | *Positive control: pBADnLIC [150ng/ul], 150ug was used | + | **Positive control: pBADnLIC [150ng/ul], 150ug was used |
| - | *Negative control: MQ | + | **Negative control: MQ |
| - | Incubated for 30min @ ice, 5min @ 37dg, 5min @ ice | + | |
| - | 800ul LB was added, and in glass tubes they were incubated (while shaking) in 37dg waterbath | + | *Incubated for 30min @ ice, 5min @ 37dg, 5min @ ice. |
| - | + | *800ul LB was added, and in glass tubes they were incubated (while shaking) in 37dg waterbath. | |
| - | + | *900ul was plated on LBA-Amp[100ug/ml]. | |
| - | O/n the plates were incubated @ 37dg.}} | + | *O/n the plates were incubated @ 37dg.}} |
Latest revision as of 12:19, 17 June 2009
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- We transformed E. coli TOP10 with the following BioBricks (first diluted in 15ul diH2O, and 2ul was taken for transformation)
- gvp (BBa_I750016, Plate 2, well 11A)
- RBS (BBa_B0034, Plate 1, well 2M)
- Terminator (BBa_B0014, Plate 2, well 24C)
- Positive control: pBADnLIC [150ng/ul], 150ug was used
- Negative control: MQ
- Incubated for 30min @ ice, 5min @ 37dg, 5min @ ice.
- 800ul LB was added, and in glass tubes they were incubated (while shaking) in 37dg waterbath.
- 900ul was plated on LBA-Amp[100ug/ml].
- O/n the plates were incubated @ 37dg.
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