Team:Newcastle/Metalsensing
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+ | ==Lab Work done== | ||
+ | {| class=wikitable border="1" | ||
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+ | ! colspan="2" | Summary of Lab Sessions for Cadmium Sensing | ||
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+ | | <center>'''Date'''</center> | ||
+ | | <center>'''Description'''</center> | ||
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+ | | '''[https://2009.igem.org/Team:Newcastle/Labwork/9_September_2009#Metal_Sensor_Team 9th September 2009]''' | ||
+ | | Arrival of ''cotC-GFP-smtA'' BioBrick - transformed this into ''DH5-alpha E. coli'' cells | ||
+ | |- | ||
+ | |} | ||
{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} |
Revision as of 20:39, 21 October 2009
Metal Sensing
Introduction
If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating.
There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA. By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation.
Modelling
BioBrick constructs
Lab Work Strategies
Other Presentations and Diagrams
Lab Work done
Summary of Lab Sessions for Cadmium Sensing | |
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9th September 2009 | Arrival of cotC-GFP-smtA BioBrick - transformed this into DH5-alpha E. coli cells |
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]