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Team:UNIPV-Pavia/Notebook/Week2Oct
From 2009.igem.org
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Revision as of 22:33, 13 October 2009
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Week from October 5th, to October 11st, 2009
Previous Week
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Next Week
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October, 5th
- Team Meeting
pH sensor
- Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
- RBS33
- A2
- pNhaA
- Overnight incubation of these three cultures at 37°C, 220 rpm.
October, 6th
pH sensor
4th experiment
- We put 50 ul into 5 ml LB NaCl 250 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four five hours.
- OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
- We diluted:
- RBS into:
- LB NaCl 250 mM + Amp pH 5,5
- LB NaCl 250 mM + Amp pH 6,6
- LB NaCl 250 mM + Amp pH 7,5
- LB NaCl 250 mM + Amp pH 8,5
- pNhaA into:
- LB NaCl 250 mM + Amp pH 5,5
- LB NaCl 250 mM + Amp pH 6,6
- LB NaCl 250 mM + Amp pH 7,5
- LB NaCl 250 mM + Amp pH 8,5
- A2 into:
- LB NaCl 250 mM + Amp pH 5,5
- LB NaCl 250 mM + Amp pH 6,6
- LB NaCl 250 mM + Amp pH 7,5
- LB NaCl 250 mM + Amp pH 8,5
- RBS into:
- We started this 24 hours experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
October, 7th
pH sensor
Qui risultati?
October, 8th
pH sensor
- Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
- RBS33
- A2
- pNhaA
- Overnight incubation of the three cultures.
October, 9th
pH sensor
5th experiment (with this experiment we try to give E.coli a pH and sodium shock to see if something happens).
- We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four four hours and a half.
- OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
- We diluted:
- RBS into:
- LB NaCl 600 mM + Amp pH 10
- LB NaCl 600 mM + Amp pH 11,2
- pNhaA into:
- LB NaCl 600 mM + Amp pH 10
- LB NaCl 600 mM + Amp pH 11,2
- A2 into:
- LB NaCl 600 mM + Amp pH 10
- LB NaCl 600 mM + Amp pH 11,2
- RBS into:
- We started this 6 hours experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
October, 10th
pH sensor
Qui risultati?
October, 11th
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Previous Week
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Next Week
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