From 2009.igem.org
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| - | =='''Contents'''==
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| | == 8-28-2009 == | | == 8-28-2009 == |
Revision as of 13:12, 15 October 2009
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8-28-2009
- Fixing cells with 4% Paraformaldehyde for microscopy on monday
8-31-2009
- Microscopy of or first fixed cells transfected with various constructs at the Nikon imaging center
- Cells tensfected with cmv-GFP, jet-GFP, cmv-jet-GFP
- The figure below shows HeLa cells transfected with GFP under a cmv promoter. The image was taken using the Nikon Eclipse 90i upright automated widefield microscope with 40X magnification:
9-2-2009
- Microscopy of cells transfected with cmv, jet, cmv-jet with GFP and an mCherry control (1:2 ratio)
- Measurement of exposure time versus average grey value to check whether there is a linear correlation between the two. This measurement was performed using a fluorescent slide that has constant fluorescence and doesn't bleech. The correlation factor was calculated to be 0.99999. Therefore we can calculate the grey values of samples with different exposure times.
- Evaluation of first microscopy measurements from 8-31-2009 using ImageJ:
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