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Team:TUDelft/Results3
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='''Results'''= | ='''Results'''= | ||
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'''''Main results''''' | '''''Main results''''' | ||
* Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans). | * Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans). | ||
| - | * Online [https://2009.igem.org/Team:TUDelft/RiboKeyLock_Generator | + | * Online [https://2009.igem.org/Team:TUDelft/RiboKeyLock_Generator lock and key generator] derived from the algorithm. |
| - | * Construction of four DNA sequences in biobrick format and pSB1A3 plasmid backbone which encode lock for medium RBS ( | + | * Construction of four DNA sequences in biobrick format and [http://partsregistry.org/wiki/index.php?title=Part:BBa_pSB1A3 pSB1A3] plasmid backbone which encode lock for medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175031 K175031]), key for the lock of medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175032 K175032]), lock for weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175029 K175029]) or key for the lock of weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175030 K175030]). |
| - | * Assembly of two composed biobricks, designed to test the functionality of the lock/key pairs constructed. | + | * Assembly of two composed biobricks ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175034 K175034] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175035 K175035]), designed to test the functionality of the lock/key pairs constructed. |
| - | * Assembly of two GFP generators under the control of PLacI with weak RBS or medium RBS. | + | * Assembly of two GFP generators under the control of PLacI with weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175033 K175033]) or medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175048 K175048]). |
[https://2009.igem.org/Team:TUDelft/Lock/Key_library Return] | [https://2009.igem.org/Team:TUDelft/Lock/Key_library Return] | ||
<br> | <br> | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 19:39, 16 October 2009
This page is still under construction
Results
Main results
- Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans).
- Online lock and key generator derived from the algorithm.
- Construction of four DNA sequences in biobrick format and [http://partsregistry.org/wiki/index.php?title=Part:BBa_pSB1A3 pSB1A3] plasmid backbone which encode lock for medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175031 K175031]), key for the lock of medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175032 K175032]), lock for weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175029 K175029]) or key for the lock of weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175030 K175030]).
- Assembly of two composed biobricks ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175034 K175034] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175035 K175035]), designed to test the functionality of the lock/key pairs constructed.
- Assembly of two GFP generators under the control of PLacI with weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175033 K175033]) or medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175048 K175048]).
