Team:Newcastle/Labwork/16 October 2009
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Revision as of 17:22, 17 October 2009
Contents |
Sending parts to the registry
Today, we prepared the synthesised bricks and placed them to the box ready to send to the parts registry.
Stochastic Switch
Suspended 5ug of the DNA in PMK backbone with 30ul of PCR water. (Final concentration:166ng/ul)
To send to the parts registry (final concentration:6.7 ng/ul in 20 ul)
0.8ul of DNA 19ul of water
AND Gate
Suspended 5ug of the DNA in pSB1AT3 backbone with 30ul of PCR water. (Final concentration:166ng/ul) To send to the parts registry (final concentration:6.7 ng/ul in 20 ul)
0.8ul of DNA 19ul of water
KinA
Measured the concentration of KinA as 64ng/ul Prepared the tube to send to the registry (6.4ng/ul)
2ul Kina in PMK 18ul of PCR water
Microscopy Test to characterize KinA
We labelled the five overnight cultures with 10ml of LB+ B. subtilis as below
- Wild type B. subtilis (- Control)
- B. subtilis transformed with pgdp-rrnb+KinA biobrick (+ Control)
- BFS867, transformed with pgdp-rrnb+KinA biobrick
- BFS840, transformed with pgdp-rrnb+KinA biobrick
- BFS82426, transformed with pgdp-rrnb+KinA biobrick
We prepared the flasks as below
- Flask1(-):5ml overnight culture 1 + 60ml LB
- Flask1(+):5ml overnight culture 1 + 60ml LB
- Flask2(-):5ml overnight culture 2 + 60ml LB + CHL
- Flask2(+):5ml overnight culture 2 + 60ml LB + CHL
- Flask3(-):5ml overnight culture 3 + 60ml LB + CHL + Em
- Flask3(+):5ml overnight culture 3 + 60ml LB + CHL + Em
- Flask4(-):5ml overnight culture 4 + 60ml LB + CHL + Em
- Flask2(+):5ml overnight culture 4 + 60ml LB + CHL + Em
- Flask5(-):5ml overnight culture 5 + 60ml LB + CHL + Em
- Flask5(+):5ml overnight culture 6 + 60ml LB + CHL + Em
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]