Team:Newcastle/Labwork/13 October 2009

From 2009.igem.org

(Difference between revisions)
(→Preparation of parst to send to the registry)
(→Preparation of parst to send to the registry)
Line 25: Line 25:
swlJ + sleB:55 ng/ul
swlJ + sleB:55 ng/ul
  To parts registry: 5.5 ng/ul in 20ul
  To parts registry: 5.5 ng/ul in 20ul
 +
 +
===Preparation of parst for sequencing===
 +
Prepared the primers for psB1AT3 (x2, prepared two sets)
 +
 +
Forward primer preparation: Total 30ul, 10pmol/ul
 +
Forward primer : 10ul (30pmol/ul)
 +
PCR water: 20ul
 +
 +
Reverse primer preparation: Total 30ul, 10pmol/ul
 +
Forward primer : 7.5ul (40pmol/ul)
 +
PCR water: 22.5ul
 +
 +
Placed into the box for sequencing parts

Revision as of 18:08, 17 October 2009

Preparation of parst to send to the registry

Measured the concentrations of the midipreps and prepared them to send to the parst registry sspB:64 ng/ul 

To parts registry: 6.4 ng/ul in 20ul

ara:34 ng/ul 

To parts registry: 3.4 ng/ul in 20ul

ara+sspB:87.5 ng/ul 

To parts registry: 8.75 ng/ul in 20ul

cotC+smtA:355.9 ng/ul 

To parts registry: 9 ng/ul in 20ul

cwlJ:76.9 ng/ul 

To parts registry: 7.69 ng/ul in 20ul

sleB:98.3 ng/ul 

To parts registry: 5 ng/ul in 20ul

swlJ + sleB:55 ng/ul 

To parts registry: 5.5 ng/ul in 20ul

Preparation of parst for sequencing

Prepared the primers for psB1AT3 (x2, prepared two sets)

Forward primer preparation: Total 30ul, 10pmol/ul 

Forward primer : 10ul (30pmol/ul)
PCR water: 20ul

Reverse primer preparation: Total 30ul, 10pmol/ul 

Forward primer : 7.5ul (40pmol/ul)
PCR water: 22.5ul
Placed into the box for sequencing parts
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