"
Team:UNIPV-Pavia/Notebook/Week4Jun
From 2009.igem.org
(Difference between revisions)
(→June, 23rd) |
(→June, 22nd) |
||
| Line 39: | Line 39: | ||
*We incubated the 5 ml cultures overnight at 37°C, 220 rpm. | *We incubated the 5 ml cultures overnight at 37°C, 220 rpm. | ||
| - | |||
| - | |||
*Wiki updating. | *Wiki updating. | ||
Revision as of 08:19, 24 June 2009
|
|
|
|
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
Week from June 22nd, to June 28th, 2009
Previous Week
|
Next Week
|
June, 22nd
- This week we planned to ligate the GFP protein generator under the control of Ptet in A4 and A6 constructs, in order to have:
- an aTc->GFP measurement system (A6-E0240 = J23100-RBS-tetR-TT-Ptet-RBS-GFP-TT) and
- a control construct that should always express GFP(A4-E0240 = RBS-tetR-TT-Ptet-RBS-GFP-TT)
- We infected 5 ml of LB + Amp with 10 ul of these glycerol stocks (to prepare samples for this week's ligations):
| K117000 | E0240 (X5) | J23118 |
| A4 | A6 |
- We incubated the 5 ml cultures overnight at 37°C, 220 rpm.
- Wiki updating.
June, 23rd
- Miniprep for: E0240 (5 samples), A4, A6, TT, J23118 and K117000
- Digestions and ligations:
- A7=J23118(S-P) + E0240(X-P)
- A8=A4(S-P) + E0240(X-P)
- A9=A6(S-P) + E0240(X-P)
- A10=TT(E-X) + K117000(E-S)
....
- Colonies grew in the LB+amp plates with the J23118-RBS-lacI-TT sample, we inoculated 5 ml in LB+amp o/n at 37°C.
June, 24th
June, 25th
June, 26th
|
Previous Week
|
Next Week
|
