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Team:TUDelft/MIntegration
From 2009.igem.org
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==Signal Plasmid== | ==Signal Plasmid== | ||
| + | The signal plasmid will contain the following features: | ||
| + | |||
| + | * oriTR - to allow transfer during conjugation | ||
| + | * constitutively expressed trbK - to block back propagation of the signal, to reduce redundant transfer, and to prevent lethal zygosis | ||
| + | * constitutively expressed trbC - if lethal zygosis turn out to be a problem | ||
| + | * constitutively expressed GFP for the green team and RFP for the red team | ||
| + | * delay device | ||
| + | ** input: the delay is triggered by the presence of the helper plasmid | ||
| + | ** output: expression of the I-SceI homing endonuclease | ||
| + | * cut sites for the I-SceI homing endonuclease | ||
==Helper Plasmid== | ==Helper Plasmid== | ||
Revision as of 21:46, 19 October 2009
Module Integration
In order for us to achieve our goal of having a relay race between GFP and RFP teams,
various parts from the the three modules must be integrated together into a single working system. What follows is a description of the requirements for the integrated system.
Signal Plasmid
The signal plasmid will contain the following features:
- oriTR - to allow transfer during conjugation
- constitutively expressed trbK - to block back propagation of the signal, to reduce redundant transfer, and to prevent lethal zygosis
- constitutively expressed trbC - if lethal zygosis turn out to be a problem
- constitutively expressed GFP for the green team and RFP for the red team
- delay device
- input: the delay is triggered by the presence of the helper plasmid
- output: expression of the I-SceI homing endonuclease
- cut sites for the I-SceI homing endonuclease
Helper Plasmid
This will consist of a modified R751 plasmid.
