"
Team:TUDelft/MIntegration
From 2009.igem.org
(Difference between revisions)
| Line 15: | Line 15: | ||
* oriTR - to allow transfer during conjugation | * oriTR - to allow transfer during conjugation | ||
| - | * constitutively expressed trbK - to block | + | * constitutively expressed trbK - to block backward propagation of the signal, to reduce redundant transfer, and to prevent lethal zygosis |
| - | * ''constitutively expressed trbC - if lethal zygosis turn out to be a problem'' | + | * ''constitutively expressed trbC - if lethal zygosis turn out to be a problem (undetermined)'' |
* constitutively expressed <font color=green>GFP</font> for the green team and <font color=red>RFP</font> for the red team | * constitutively expressed <font color=green>GFP</font> for the green team and <font color=red>RFP</font> for the red team | ||
* delay device | * delay device | ||
| Line 26: | Line 26: | ||
==Helper Plasmid== | ==Helper Plasmid== | ||
| - | + | The helper plasmid will consist of a modified R751 plasmid with the following features: | |
* trbK knockout to allow cells containing the helper plasmid to conjugate | * trbK knockout to allow cells containing the helper plasmid to conjugate | ||
* oriTR knockout to prevent transfer of the helper plasmid during conjugation | * oriTR knockout to prevent transfer of the helper plasmid during conjugation | ||
| - | * ''trbC knockout to prevent lethal zygosis'' | + | * ''trbC knockout to prevent lethal zygosis (undetermined)'' |
* constitutively expressed trigger for the delay device | * constitutively expressed trigger for the delay device | ||
| + | |||
| + | ==Future Applications== | ||
| + | |||
| + | We foresee many interesting applications becoming available once the full system is operational. These include everything from logic circuits to the generation of spatiotemporal patters: | ||
| + | |||
| + | [[Image:TUD09ring.gif|frame]] | ||
| + | |||
[[Team:TUDelft | <<< Back Home]] | [[Team:TUDelft | <<< Back Home]] | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 22:05, 19 October 2009
Module Integration
In order for us to achieve our goal of having a relay race between GFP and RFP teams,
various parts from the the three modules must be integrated together into a single working system. What follows is a description of the requirements for the integrated system.
Signal Plasmid
The signal plasmid will contain the following features:
- oriTR - to allow transfer during conjugation
- constitutively expressed trbK - to block backward propagation of the signal, to reduce redundant transfer, and to prevent lethal zygosis
- constitutively expressed trbC - if lethal zygosis turn out to be a problem (undetermined)
- constitutively expressed GFP for the green team and RFP for the red team
- delay device
- input: the delay is triggered by the presence of the helper plasmid
- output: expression of the I-SceI homing endonuclease
- cut sites for the I-SceI homing endonuclease
- a low copy backbone to allow the endonuclease to destroy the signal plasmid
Helper Plasmid
The helper plasmid will consist of a modified R751 plasmid with the following features:
- trbK knockout to allow cells containing the helper plasmid to conjugate
- oriTR knockout to prevent transfer of the helper plasmid during conjugation
- trbC knockout to prevent lethal zygosis (undetermined)
- constitutively expressed trigger for the delay device
Future Applications
We foresee many interesting applications becoming available once the full system is operational. These include everything from logic circuits to the generation of spatiotemporal patters:
