Team:KU Seoul/Details

From 2009.igem.org

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{|style=  "background:#FFCC33;" align="center"
{|style=  "background:#FFCC33;" align="center"
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=== The Experiments ===
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== The Experiments ==
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== Materials ==
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=== Materials ===
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#Backbone Plasmids  
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*Backbone Plasmids  
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##Plasmid pSB3C5 with part BBa_J04450 : 2009 Kit Plate 1 [5C]
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**Plasmid pSB3C5 with {{part|BBa_J04450}} : 2009 Kit Plate 1 [5C]
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##Plasmid pSB3T5 with part BBa_J04450 : 2009 Kit Plate 1 [9C]
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**Plasmid pSB3T5 with {{part|BBa_J04450}} : 2009 Kit Plate 1 [9C]
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*Promoters
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#Promoters
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**Promoter arsR [ParsR], zntA [Pznt] and yodA [PyodA](ref) originated from genomic DNA of Escherichia coli XL-1 Blue
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##Promoter arsR [ParsR], zntA [Pznt] and yodA [PyodA](ref) originated from genomic DNA of Escherichia coli XL-1 Blue
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*Protein Coding Sequences
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**{{part|BBa_E0044|Green fluorescent protein(BBa_E0044)}}  : 2009 Kit Plate 1 [14G]
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#Protein Coding Sequences
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**{{part|BBa_E1010|Red fluorescent protein(BBa_E1010)}} : 2009 Kit Plate 1 [18F]
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##Green fluorescent protein [BBa_E0044] : 2009 Kit Plate 1 [14G]
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**Aryl acylamidase protein(ref) : [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=227462445&dopt=GenBank new biological part]
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##Red fluorescent protein [BBa_E1010] : 2009 Kit Plate 1 [18F]
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*Primers
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##Aryl acylamidase protein(ref) : [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=227462445&dopt=GenBank new biological part]
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{| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"
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! Template
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! Primer
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! Sequence
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! Length(bp)
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|-
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|rowspan="2"|Plasmid pSB3C5 || pSB3C5_F ||gctgctgttTAATAAtactagtagcggccgctgc ||align="center" |34
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|-
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|pSB3C5(+Pars)_R ||taataggtgtgaattttgagttggCtctagaagcggccgcga ||align="center" |42
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|-
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|rowspan="2"|Plasmid pSB3T5 ||pSB3T5_F ||gacgaaaactacgctgctgctgttTAATAAtactagtagcggccgctgc ||align="center" |49
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|-
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|pSB3T5_R ||GTCGGCAATATGAAGctctagaagcggccgcga ||align="center" |33
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|-
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|rowspan="2"|Promoter yodA ||PyodA_F ||cggccgcttctagagCTTCATATTGCCGACAAAGTACG ||align="center" |38
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|-
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|PyodA_R ||ATGTGACTTACCCATaacAGTTTCCTCCAGAGTCATTG ||align="center" |38
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|-
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|rowspan="4"|Green fluorescent protein ||rowspan="2"|GR(+Pars)_F || aattcacacctattaccttcctctgcacttacacattcgttaagtcatatATGc ||rowspan="2" align="center" |78
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|-
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|gtaaaggagaagaacttttcactg
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|-
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|rowspan="2"|GR(+Pznt)_R || ctggagtcgactccagagtcaagttttatcagagatacagcgagcggacg ||rowspan="2" align="center" |84
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|-
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|TCTAGTttattaaacagcagcagcgtagttttcg
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|-
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|rowspan="4"|Red fluorescent protein
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|rowspan="2"|RF(+Pznt)_F || tgataaaacttgactctggagtcgactccagagtgtatccttcggttaatATG ||rowspan="2" align="center" |75
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|-
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|gcttcctccgaagacgttatca
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|-
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|rowspan="2"|RF(+AAV tag)_R || TTATTAaacagcagcagcgtagttttcgtcgtttgctgcAgcaccggtgg ||rowspan="2" align="center" |59
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|-
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|agtgacgac
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|-
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|rowspan="3"|Aryl acylamidase
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|AMD_F || CTGGAGGAAACTGTTatgGGTAAGTCACATTCGCCAG ||align="center" |37
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|-
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|rowspan="2"|AMD(+AAV tag)_R || TTATTAaacagcagcagcgtagttttcgtcgtttgctgcCAGGGGC ||rowspan="2" align="center" |55
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|-
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|CGTCCGGCG
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|}
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*Chemicals
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**Acetaminophen (Sigma)
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**CdCl2∙H2O (Junsei)
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**ZnCl2 (Sigma)
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**KH2AsO3 (Sigma)
 +
**Luria-Bertani broth & Bacto agar (Difco)
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**o-Cresol (Sigma)
 +
**CuSO4∙5H2O (Sigma)
|}
|}

Revision as of 05:38, 20 October 2009






The Experiments

Materials

  • Backbone Plasmids
    • Plasmid pSB3C5 with [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] : 2009 Kit Plate 1 [5C]
    • Plasmid pSB3T5 with [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] : 2009 Kit Plate 1 [9C]
  • Promoters
    • Promoter arsR [ParsR], zntA [Pznt] and yodA [PyodA](ref) originated from genomic DNA of Escherichia coli XL-1 Blue
  • Protein Coding Sequences
    • [http://partsregistry.org/Part:BBa_E0044 Green fluorescent protein(BBa_E0044)]  : 2009 Kit Plate 1 [14G]
    • [http://partsregistry.org/Part:BBa_E1010 Red fluorescent protein(BBa_E1010)] : 2009 Kit Plate 1 [18F]
    • Aryl acylamidase protein(ref) : [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=227462445&dopt=GenBank new biological part]
  • Primers
Template Primer Sequence Length(bp)
Plasmid pSB3C5 pSB3C5_F gctgctgttTAATAAtactagtagcggccgctgc 34
pSB3C5(+Pars)_R taataggtgtgaattttgagttggCtctagaagcggccgcga 42
Plasmid pSB3T5 pSB3T5_F gacgaaaactacgctgctgctgttTAATAAtactagtagcggccgctgc 49
pSB3T5_R GTCGGCAATATGAAGctctagaagcggccgcga 33
Promoter yodA PyodA_F cggccgcttctagagCTTCATATTGCCGACAAAGTACG 38
PyodA_R ATGTGACTTACCCATaacAGTTTCCTCCAGAGTCATTG 38
Green fluorescent protein GR(+Pars)_F aattcacacctattaccttcctctgcacttacacattcgttaagtcatatATGc 78
gtaaaggagaagaacttttcactg
GR(+Pznt)_R ctggagtcgactccagagtcaagttttatcagagatacagcgagcggacg 84
TCTAGTttattaaacagcagcagcgtagttttcg
Red fluorescent protein RF(+Pznt)_F tgataaaacttgactctggagtcgactccagagtgtatccttcggttaatATG 75
gcttcctccgaagacgttatca
RF(+AAV tag)_R TTATTAaacagcagcagcgtagttttcgtcgtttgctgcAgcaccggtgg 59
agtgacgac
Aryl acylamidase AMD_F CTGGAGGAAACTGTTatgGGTAAGTCACATTCGCCAG 37
AMD(+AAV tag)_R TTATTAaacagcagcagcgtagttttcgtcgtttgctgcCAGGGGC 55
CGTCCGGCG
  • Chemicals
    • Acetaminophen (Sigma)
    • CdCl2∙H2O (Junsei)
    • ZnCl2 (Sigma)
    • KH2AsO3 (Sigma)
    • Luria-Bertani broth & Bacto agar (Difco)
    • o-Cresol (Sigma)
    • CuSO4∙5H2O (Sigma)
Retrieved from "http://2009.igem.org/Team:KU_Seoul/Details"