Team:Warsaw/Calendar-Main/11 May 2009
From 2009.igem.org
(Difference between revisions)
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<li>Electrophoretic separation on 1% agarose gel</li> | <li>Electrophoretic separation on 1% agarose gel</li> | ||
+ | <br/> | ||
<p>Results:</P> | <p>Results:</P> | ||
<img src="https://static.igem.org/mediawiki/2009/b/b8/2009.05.11_-_PCR_pho%2C_mgtc_i_inv_opisany.jpg"/> | <img src="https://static.igem.org/mediawiki/2009/b/b8/2009.05.11_-_PCR_pho%2C_mgtc_i_inv_opisany.jpg"/> | ||
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<ul> | <ul> | ||
<li>Gel (from left)</li> | <li>Gel (from left)</li> | ||
- | 1. GeneRuler DNA Ladder Mix #SM0333 (Fermentas) | + | </ul> |
- | 2. pho | + | <ol> |
- | 3. pho control - | + | <li>1. GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li> |
- | 4. mgtc | + | <li>2. pho</li> |
- | 5. mgtc control - | + | <li>3. pho control -</li> |
- | 6. inv | + | <li>4. mgtc</li> |
- | 7. inv control - | + | <li>5. mgtc control -</li> |
- | + | <li>6. inv</li> | |
- | Notes: | + | <li>7. inv control -</li> |
- | No product. Check the template (Salmonella), polymerase (KNGiE) and dNTPs. | + | </ol> |
+ | </var> | ||
+ | <br /> | ||
+ | <p>Notes:</p> | ||
+ | <ul> | ||
+ | <li>No product. Check the template (Salmonella), polymerase (KNGiE) and dNTPs.</li> | ||
+ | </ul> | ||
+ | <br /> | ||
</html> | </html> | ||
Revision as of 19:54, 25 June 2009
PCR pho, mgtc and inv
Kama
Tasks:
- Amplification of pho, mgtc, inv
Methods:
PCR mixture's composition:
2,5ul pfu buffer (Fermentas), 2,5ul MgSO4 (Fermentas), 1,5ul primers, 1ul dNTPs (10 mM), 1ul template, 0,5ul pfu turbo polymerase (KNGiE), solution was topped up with H2O to 25ul.
- PCR programs:
pho
2min30s 95C
3x(30s 95C, 35s 56C, 3min30s 72C)
28x(30s 95C, 35s 61C, 3min30s 72C)
10min 72C
~ 7C
mgtc
1min30s 95C
3x(30s 95C, 35s 48C, 1min 72C)
28x(30s 95C, 35s 58C, 1min 72C)
10min 72C
~ 7C
inv
4min 95C
31x(30s 95C, 1min 53C , 4min15s 72C)
10min 72C
~ 7C
- Electrophoretic separation on 1% agarose gel
- Gel (from left)
- 1. GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
- 2. pho
- 3. pho control -
- 4. mgtc
- 5. mgtc control -
- 6. inv
- 7. inv control -
- No product. Check the template (Salmonella), polymerase (KNGiE) and dNTPs.
Results:
Notes:
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